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The present study provides a novel mechanism by which miRNA\u201022 positively regulates metal\u2010regulatory transcription factor 1 (<jats:italic>Mtf1<\/jats:italic>) in the nuclei of neurons in the dorsal horn of the spinal cord. We found that miRNA\u201022 was significantly increased in the dorsal horn of mice with either inflammatory pain induced by plantar injection of complete Freund\u2019s adjuvant (CFA) or neuropathic pain induced by unilateral sciatic nerve chronic constrictive injury (CCI). Knocking down or blocking miRNA\u201022 alleviated CFA\u2010induced mechanical allodynia and heat hyperalgesia, whereas overexpressing miRNA\u201022 produced pain\u2010like behaviors. Mechanistically, the increased miRNA\u201022 binds directly to the <jats:italic>Mtf1<\/jats:italic> promoter to recruit RNA polymerase II and elevate <jats:italic>Mtf1<\/jats:italic> expression. The increased <jats:italic>Mtf1<\/jats:italic> subsequently enhances spinal central sensitization, as evidenced by increased expression of p\u2010ERK1\/2, GFAP, and c\u2010Fos in the dorsal horn. Our findings suggest that the miRNA\u201022\u2013<jats:italic>Mtf1<\/jats:italic> signaling axis in the dorsal horn plays a critical role in the induction and maintenance of inflammatory pain. This signaling pathway may be a promising therapeutic target in inflammatory pain.<\/jats:p>","DOI":"10.1155\/2022\/8622388","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T00:05:53Z","timestamp":1644537953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["miRNA\u201022 Upregulates <i>Mtf1<\/i> in Dorsal Horn Neurons and Is Essential for Inflammatory Pain"],"prefix":"10.1155","volume":"2022","author":[{"given":"Ling-Yun","family":"Hao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengjun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoqiao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kehui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runa","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouya","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Li","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-5129","authenticated-orcid":false,"given":"Zhiqiang","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws330"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf8924"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3474-15.2016"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2262-16.2016"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1631-18.2018"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1980-10.2010"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.249"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0921-11.2011"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09783"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21218362"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5346-13.2014"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.1627"},{"key":"e_1_2_9_13_2","first-page":"491","article-title":"MicroRNA-22 regulates the proliferation, drug sensitivity and metastasis of human glioma cells by targeting SNAIL1. 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