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Illegally trafficked snake venom might be misidentified with other drugs of abuse, and sometimes, the species of venom\u2010yielding snake cannot be verified. Snake venoms from medically important snake species, <jats:italic>Naja naja<\/jats:italic> and <jats:italic>Daboia russelii,<\/jats:italic> were procured from Irula Snake Catcher\u2019s Society, Tamil Nadu, India. Comparative analyses of both venoms were carried out using SDS\u2010PAGE, LC\u2010MS\/MS, ICP\u2010MS, and mtDNA analysis. The protein concentration of <jats:italic>Naja naja<\/jats:italic> and <jats:italic>Daboia russelii<\/jats:italic> venoms was 76.1% and 83.9%, respectively. SDS analysis showed a distinct banding pattern of both venoms. LC\u2010MS\/MS results showed proteins and toxins from 12 to 14 protein families in <jats:italic>Naja naja<\/jats:italic> and <jats:italic>Daboia russelii<\/jats:italic> venoms. Elemental analysis using ICP\u2010MS showed a different profile of some elements in both venoms. mtDNA analysis of venoms using universal primers against <jats:italic>Cyt b<\/jats:italic> gene showed homology with sequence of <jats:italic>Naja naja<\/jats:italic> and <jats:italic>Daboia russelii<\/jats:italic> genes. The study proposed a template of various conventional and advanced molecular and instrumental techniques with their pros and cons. The template can be used by forensic science laboratories for detection, screening, and confirmatory analysis of suspected venoms of snakes. Clubbing of various techniques can be used to confirm the identification of species of snake from which the alleged venom was milked. The results can be helpful in framing charge\u2010sheets against accused of illegal venom trafficking and can also be used to verify the purity and quality of commercially available snake venoms.<\/jats:p>","DOI":"10.1155\/2022\/8644993","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T13:50:07Z","timestamp":1654264207000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative Snake Venom Analysis for Facilitating Wildlife Forensics: A Pilot Study"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3569-3287","authenticated-orcid":false,"given":"Saurabh","family":"Bhargava","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7123-4443","authenticated-orcid":false,"given":"Kiran","family":"Kumari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra Kumar","family":"Sarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4209-7659","authenticated-orcid":false,"given":"Rajvinder","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-38.2.239"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-010-0317-y"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1556-4029.2011.02049.x"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2020.108601"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61754-2"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9090290"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2011.621940"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0516-4"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7610-9"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_1078_14"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.21740"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22470"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9120392"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-2018-3"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2018.09.011"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.4331\/wjbc.v10.i1.17"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2019.00218"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/08897077.2011.540482"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.4103\/2229-5151.119202"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.5958\/0974-4568.2019.00020.6"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins6051667"},{"key":"e_1_2_12_22_2","unstructured":"WeirD. 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