{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:18:41Z","timestamp":1789474721714,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T00:00:00Z","timestamp":1642550400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Osaka Medical and Pharmaceutical University"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Nutrition and Metabolism"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    Sulforaphane and allyl isothiocyanate, naturally occurring isothiocyanates, have been reported to inhibit adipocyte differentiation, but little is known about how they compare in terms of their potency and mechanism of action. In the present study, we compared the effects of sulforaphane and allyl isothiocyanate on the differentiation of 3T3\u2010L1 preadipocytes. A mixture of insulin, dexamethasone, and 3\u2010isobutyl\u20101\u2010methylxanthine was used to establish a differentiation medium. We found that, at a concentration as low as one\u2010tenth that of allyl isothiocyanate, sulforaphane reduced triacylglycerol levels, lipid\u2010filled adipocyte quantity, and mRNA and protein levels of CCAAT\u2010enhancer\u2010binding protein\n                    <jats:italic>\u03b1<\/jats:italic>\n                    (C\/EBP\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) and peroxisome proliferator\u2010activated receptor\n                    <jats:italic>\u03b3<\/jats:italic>\n                    (PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    ). These results suggested that sulforaphane may be a more potent adipocyte differentiation inhibitor than allyl isothiocyanate. Our results may provide insight into possible strategies for the prevention of obesity and related conditions.\n                  <\/jats:p>","DOI":"10.1155\/2022\/8705163","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T15:20:08Z","timestamp":1642605608000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Effects of Sulforaphane and Allyl Isothiocyanate on 3T3\u2010L1 Adipogenesis"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3563-2190","authenticated-orcid":false,"given":"Satoru","family":"Sakuma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kana","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risa","family":"Kitahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaho","family":"Tsujimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kishiko","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Hoshino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohko","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0127-9571","authenticated-orcid":false,"given":"Keiichiro","family":"Okuhira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,19]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/nejmp068177"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/nejmhpr060227"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2007.100156"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35007508"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3391-5"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201870079"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470771532.ch6"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0348-5795-6_10"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2010.11.005"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1617202"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1039\/c8fo00763b"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.10-44"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.58.1"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2017.05.036"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(00)01655-0"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.119.117887"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2015.09.015"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.2217\/fon.11.145"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-014-9305-9"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25092093"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172398899"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2006.10.004"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2012.04.003"}],"container-title":["Journal 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