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It is important to identify drugs to resist osteoclastogenesis to manage the bone-loss disorders. Huangqi Sanxian decoction (HQSXD) is utilized for the treatment of postmenopausal osteoporosis (PMOP) for a long history in East Asia. This work aimed to examine HQSXD\u2019s activity in OC differentiation. Based on staining with tartrate-resistant acid phosphatase (TRAP), it was found that HQSXD suppressed OC generation under the induction of RANKL produced in the bone marrow-derived monocytes\/macrophages (BMMs), with no cytotoxic effect. Later analysis like molecular exploration and network pharmacology (NP) suggested the role of HQSXD in suppressing genes associated with osteoclastogenesis via PI3K\/Akt-mediated mechanism dose-dependently. This work might illustrate the molecular pharmacological mechanism involved in HQSXD\u2019s effect on treating OC-associated disorders. Moreover, NP was found to modernize traditional Chinese medicine (TCM) research.<\/jats:p>","DOI":"10.1155\/2022\/8769531","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T23:20:11Z","timestamp":1654730411000},"page":"1-11","source":"Crossref","is-referenced-by-count":1,"title":["Mechanism of Huangqi Sanxian Decoction Inhibiting Osteoclast Differentiation Based on Network Pharmacology"],"prefix":"10.1155","volume":"2022","author":[{"given":"Jian-Xin","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Guangdong Medical University, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China"},{"name":"Huizhou Women\u2019s and Children\u2019s Hospital Affiliated to Guangdong Medical University, No. 10, Daling Road, Huicheng District, Huizhou 516001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Qun","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Guangdong Medical University, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze-Hao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Guangdong Medical University, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Guangdong Medical University, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9697-5897","authenticated-orcid":true,"given":"Zhi-Kun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Guangdong Medical University, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.063032"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2008.07.016"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2016.02.003"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2014.06.001"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-017-0373-1"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kew430"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2005.11.007"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401285"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513500306"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.03.018"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26646"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.08.004"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m805280200"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.10.117"},{"key":"15","first-page":"21","article-title":"Bone resorption and matrix metalloproteinase-9 (MMP-9) secretion are impaired in MMP9 antisense-DNA treated osteoclast cultures in vitro","volume":"30","author":"J. 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