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The present work aims to modify the composite coating layer properties by using two different types of bioactive reinforcing materials (biotin and hydroxyapatite) particles in different percentages (5% and 10%). Coatings were applied onto commercially pure Ti, SS 316\u2009L, and SS 304 substrates by the dip-coating method. Characterization of samples includes microstructure observation by field emission scanning electron microscopy (FE-SEM), contact angle measurement (wettability), and MTT. The results show the addition of metallic particles (bioparticles) (hydroxyapatite particles, biotin) at 5 Vol. % improved the whole properties of composite materials. Using different particles\u2019 scale size aids to enhance the combinations in the alginate matrix producing a dual effect on composite film properties. In addition, the inclusion of metallic particles has to increase the wettability by reducing the contact angle. At the same time, MTT graphs revealed that after 3 days of exposure in MG-63 cells, 316\u2009L SS alloys\u2019 surface following pack adhesion became more active.<\/jats:p>","DOI":"10.1155\/2022\/8802111","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T20:20:07Z","timestamp":1664223607000},"page":"1-11","source":"Crossref","is-referenced-by-count":15,"title":["Comparative Study of Biotin and Hydroxyapatite on Biological Properties of Composite Coating"],"prefix":"10.1155","volume":"2022","author":[{"given":"Qahtan A.","family":"Hamad","sequence":"first","affiliation":[{"name":"Materials Engineering Department, University of Technology, Baghdad, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatimah J.","family":"Al-Hasani","sequence":"additional","affiliation":[{"name":"Materials Engineering Department, University of Technology, Baghdad, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4404-7057","authenticated-orcid":true,"given":"Noor K.","family":"Faheed","sequence":"additional","affiliation":[{"name":"Department of Petroleum Engineering, University of Misan, College of Engineering, Misan, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","volume-title":"Biomedical Engineering Fundamentals","author":"D. 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