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However, the effect of (R)\u2010sal on repeated dextran sulfate sodium (DSS)\u2010induced chronic colitis has not yet been investigated. In this study evaluated the potential effect of (R)\u2010, (S)\u2010, and (RS)\u2010sal in mice with repeated DSS\u2010induced chronic colitis and investigated the underlying mechanisms. Here, we verified that chronic colitis was significantly attenuated by (R)\u2010sal, which was evidenced by notably mitigated body weight loss, disease activity index (DAI), splenomegaly, colonic lengths shortening, and histopathological scores. (R)\u2010sal treatment noticeably diminished the levels of inflammatory cytokines (such as TNF\u2010<jats:italic>\u03b1<\/jats:italic>, IL\u20106, IL\u20101<jats:italic>\u03b2<\/jats:italic>, and IFN\u2010<jats:italic>\u03b3<\/jats:italic>). Notably, the efficacy of (R)\u2010sal was better than that of (RS)\u2010sal. Further research revealed that (R)\u2010sal mitigated colonic CD4 leukocyte infiltration, decreased NF\u2010<jats:italic>\u03ba<\/jats:italic>B signaling pathway activation, improved the Nrf\u20102\/HO\u20101 signaling pathway, and increased the expression of ZO\u20101 and occludin. In addition, (R)\u2010sal suppressed the levels of TGF\u2010<jats:italic>\u03b2<\/jats:italic>1, <jats:italic>\u03b1<\/jats:italic>\u2010SMA, and collagen in mice with chronic colitis. Furthermore, the 16S rDNA sequences analyzed of the intestinal microbiome revealed that (R)\u2010sal could mitigate the intestinal microbiome structure and made it more similar to the control group, which mainly by relieving the relative abundance of pathogens (such as Bacteroides) and increasing the relative abundance of probiotics (such as Akkermansia). Therefore, (R)\u2010sal ameliorates repeated DSS\u2010induced chronic colitis in mice by improving inflammation, suppressing oxidative stress, mitigating intestinal barrier function, relieving intestinal fibrosis, and regulating the intestinal microbiome community. These results indicate that (R)\u2010sal maybe a novel treatment alternative for chronic colitis.<\/jats:p>","DOI":"10.1155\/2022\/9318721","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T03:05:09Z","timestamp":1644289509000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["The Attenuation of Chronic Ulcerative Colitis by (R)\u2010salbutamol in Repeated DSS\u2010Induced Mice"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4215-182X","authenticated-orcid":false,"given":"Liangjun","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihua","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1313-369X","authenticated-orcid":false,"given":"Shanping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9553-2848","authenticated-orcid":false,"given":"Wen","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,7]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.128"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0b013e328354be1e"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365528909091339"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11938-018-0175-1"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)32448-0"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(17)30004-3"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000584"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000313"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-016-1207-z"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2018-317571"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0306-4"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2432416"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20243"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2010.01473.x"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjms.2018.01.002"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0338OC"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14780"},{"key":"e_1_2_10_18_2","article-title":"(R)-salbutamol improves imiquimod-induced psoriasis-like skin dermatitis by regulating the Th17\/Tregs balance and glycerophospholipid metabolism","volume":"9","author":"Liu F.","year":"2020","journal-title":"Cell"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.044"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1525s104"},{"key":"e_1_2_10_21_2","first-page":"238","article-title":"Clinicopathologic study of dextran sulfate sodium experimental murine colitis","volume":"69","author":"Cooper H. 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