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Traditional Chinese medicine (TCM) emphasizes treatment based on syndrome differentiation. This study aimed to clarify the characteristics of DNA methylation expression profiles in peripheral blood mononuclear cells (PBMCs) in patients with psoriasis and analyze the differences in these profiles among different TCM syndromes of psoriasis in order to provide a material basis for the diversity of these syndromes. Methods. Blood samples were collected from 32 participants, including 14 patients with psoriatic blood heat syndrome (BHS), 12 patients with psoriatic blood stasis syndrome (BSS), and 6 healthy controls. PBMCs were extracted and subjected to DNA quality inspection. An Illumina Human Methylation 850k chip was used to sequence each group of samples. According to gene annotation classification together with CpG island annotation classification, the differentially methylated regions between sample groups were screened, while Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were applied to perform functional analyses of DMGs. Finally, the DMGs closely correlating with psoriatic severity were screened using Spearman\u2019s correlation analysis. Results. Compared with normal controls, patients with psoriasis showed an overall trend of hypermethylation. In psoriasis, the differential methylation probes were mainly distributed on gene body region on the genome, while those in CpG regions were mainly distributed in CpG islands. Compared with healthy controls, the overall trends in methylation were similar in psoriatic BHS and BSS patients compared to healthy controls. However, bioinformatic analysis revealed different functions of DMGs. We also found that the methylation levels of TRIM14 and PRDM16 were closely correlated with PASI scores and could serve as potential biomarkers to assess the severity of psoriasis. Conclusions. Our study, for the first time, indicated the possible involvement of DNA methylation in regulating the characteristics of TCM syndromes of psoriasis, providing a new direction for research into TCM psoriatic syndromes.<\/jats:p>","DOI":"10.1155\/2022\/9343285","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T02:35:25Z","timestamp":1663641325000},"page":"1-20","source":"Crossref","is-referenced-by-count":5,"title":["DNA Methylation Expression Profile of Blood Heat Syndrome and Blood Stasis Syndrome in TCM Psoriasis"],"prefix":"10.1155","volume":"2022","author":[{"given":"Meng","family":"Xing","sequence":"first","affiliation":[{"name":"Department of Dermatology, Shaanxi Hospital of Traditional Chinese Medicine, Xi\u2019an 710003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China"},{"name":"Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Kuai","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China"},{"name":"Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Ru","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China"},{"name":"Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojie","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China"},{"name":"Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoninng","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shaanxi Hospital of Traditional Chinese Medicine, Xi\u2019an 710003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8607-8874","authenticated-orcid":true,"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China"},{"name":"Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200443, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2525-9679","authenticated-orcid":true,"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China"},{"name":"Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1689"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-018-0541-9"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2013.07.002"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.04.128"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1655"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1038\/ng1909"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2013.110"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1080\/09546634.2020.1789048"},{"key":"9","first-page":"61","article-title":"Modern literature research on the characteristics of psoriasis vulgaris syndrome distribution in different times","volume":"3","author":"X. 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