{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:06:28Z","timestamp":1791234388991,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":258,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MH186"],"award-info":[{"award-number":["ZR2020MH186"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Carbon monoxide\u2010releasing molecule\u20103 (CORM\u20103) is a water\u2010soluble complex which has the ability to release carbon monoxide (CO). The study is aimed at investigating the epidemiological characters and effects of CORM\u20103 on tongue squamous cell carcinoma (TSCC) cells and the mechanisms involved. Firstly, CAL27 and SCC4 were treated with CORM\u20103 or iCORM\u20103. The proliferation, migration, and invasion of cells were separately evaluated by CCK\u20108, scratch assay, and transwell assay. We found that the optimal concentration of CORM\u20103 on the proliferation of CAL27 and SCC4 cells was 400\u2009<jats:italic>\u03bc<\/jats:italic>M, and CORM\u20103 was significantly inhibited the proliferation, migration, and invasion of TSCC cells. Meanwhile, CORM\u20103 increased the protein expression of HO\u20101 detected by western blot. Short\u2010hairpin RNAs (shRNAs) were constructed to manipulate the expression of HO\u20101 in CAL27 and SCC4 cells. Then, rescue assays were conducted to explore the reversion effect of shHO\u20101 on the CORM\u20103 function. Mechanistically, CORM\u20103 decreased the protein of Keap1 expression as well as increased Nrf2 expression. Upregulation of E\u2010cadherin was observed, as well as the downregulation of N\u2010cadherin expression significantly. The antitumor effect of CORM\u20103 was used to xenograft tumor in nude mice for further investigation <jats:italic>in viv<\/jats:italic>o, and the result showed that CORM\u20103 significantly suppressed tumor growth in xenograft nude mice. These data suggest that CORM\u20103 acts as a tumor suppressor by regulating the Keap1\/Nrf2\/HO\u20101 signaling pathway in TSCC, which provides a potential chemotherapeutic strategy for TSCC.<\/jats:p>","DOI":"10.1155\/2022\/9418332","type":"journal-article","created":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T01:50:08Z","timestamp":1663379408000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Carbon Monoxide\u2010Releasing Molecule\u20103 Suppresses the Malignant Biological Behavior of Tongue Squamous Cell Carcinoma via Regulating Keap1\/Nrf2\/HO\u20101 Signaling Pathway"],"prefix":"10.1155","volume":"2022","author":[{"given":"Yan","family":"Dai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9526-126X","authenticated-orcid":false,"given":"Hui","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2018.01.008"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-016-4150-y"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djq366"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2016.157"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612823666170413122439"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.09.112"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2013.10.002"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-012-9612-7"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.2174\/1573406415666191206102452"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13537"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-020-00659-8"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.05.002"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-019-0435-2"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1958174"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-011-9230-4"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080315"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5184"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2018.1473571"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26223"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000084381.86567.08"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2374"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010047"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3228"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612033453785"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3129"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1159\/000494891"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3437"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5521196"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0080-4"},{"key":"e_1_2_9_30_2","first-page":"3299","article-title":"The Cap\u2018n\u2019Collar basic leucine zipper transcription factor Nrf2 (NF-E2 p45-related factor 2) controls both constitutive and inducible expression of intestinal detoxification and glutathione biosynthetic enzymes","volume":"61","author":"McMahon M.","year":"2001","journal-title":"Cancer Research"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-018-0262-x"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14241"},{"key":"e_1_2_9_33_2","article-title":"The KEAP1-NRF2 system as a molecular target of cancer treatment","volume":"13","author":"Taguchi K.","year":"2021","journal-title":"Cancers"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2017.00085"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300931200"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.07.002"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X16500580"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-020-00178-0"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2022\/9418332.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2022\/9418332.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2022\/9418332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:35:32Z","timestamp":1735691732000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2022\/9418332"}},"subtitle":[],"editor":[{"given":"Qiang","family":"Huo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1155\/2022\/9418332"],"URL":"https:\/\/doi.org\/10.1155\/2022\/9418332","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2022-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9418332"}}