{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T18:36:46Z","timestamp":1774550206032,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["202300410460"],"award-info":[{"award-number":["202300410460"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["SBGJ202002077"],"award-info":[{"award-number":["SBGJ202002077"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["20XTZX08017"],"award-info":[{"award-number":["20XTZX08017"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Medical Science and Technology Project of Henan Province","award":["202300410460"],"award-info":[{"award-number":["202300410460"]}]},{"name":"Medical Science and Technology Project of Henan Province","award":["SBGJ202002077"],"award-info":[{"award-number":["SBGJ202002077"]}]},{"name":"Medical Science and Technology Project of Henan Province","award":["20XTZX08017"],"award-info":[{"award-number":["20XTZX08017"]}]},{"name":"Collaborative Innovation Major Project of Zhengzhou","award":["202300410460"],"award-info":[{"award-number":["202300410460"]}]},{"name":"Collaborative Innovation Major Project of Zhengzhou","award":["SBGJ202002077"],"award-info":[{"award-number":["SBGJ202002077"]}]},{"name":"Collaborative Innovation Major Project of Zhengzhou","award":["20XTZX08017"],"award-info":[{"award-number":["20XTZX08017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2022,8,9]]},"abstract":"<jats:p>Diffuse large B cell lymphoma (DLBCL) is one of the most usual types of adult lymphoma with heterogeneousness in histological morphology, prognosis, and clinical indications. Prior to this, several studies were carried out to determine the DLBCL subtype based on the analysis of the genome profile. However, classification based on assessment of genes related to the immune system has limited clinical significance for DLBCL. We systematically explored the DLBCL gene expression dataset and provided publicly available clinical information on patients with GEO. In this research, 928 DLBCL samples were applied, and we calculated 29 immune-related genomes\u2019 enrichment levels in each sample and stratified them into high immunity (Immunity_H, <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>n<\/a:mi>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>135<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>, 28.7%), moderate immunity (Immunity_M, <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>n<\/c:mi>\n                        <c:mo>=<\/c:mo>\n                        <c:mn>135<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>, 28.7%), and low immunity (Immunity_L, <jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>n<\/e:mi>\n                        <e:mo>=<\/e:mo>\n                        <e:mn>12<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>, 2.6%) that was based on ssGSEA score. The ESTIMATE algorithm was used to calculate stromal scores (range 586.88 to 1982.43), immune scores, estimated scores (range 2,618.2 to 8,098.14), and tumor purity (range 0.216 to 0.976). All of them were significantly correlated with immune subtypes (Kruskal-Wallis test, <jats:inline-formula>\n                     <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mi>p<\/g:mi>\n                        <g:mo>&lt;<\/g:mo>\n                        <g:mn>0.001<\/g:mn>\n                     <\/g:math>\n                  <\/jats:inline-formula>). At the same time, the correlation of related genes was analyzed by immunohistochemistry staining. In addition, DLBCL cells were cultured in transfected and in vitro with siRNA to verify correlation analysis and gene expression. Finally, human peripheral blood lymphocytes were incubated with DLBCL cells and stained. Flow cytometry was applied to analyze genes\u2019 influence on immune function. By analysis, immune checkpoint and HLA gene expression levels were higher in the Immunity_H group (Kruskal-Wallis test, <jats:inline-formula>\n                     <i:math xmlns:i=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\">\n                        <i:mi>p<\/i:mi>\n                        <i:mo>&lt;<\/i:mo>\n                        <i:mn>0.05<\/i:mn>\n                     <\/i:math>\n                  <\/jats:inline-formula>). The levels of Tfhs (follicular helper T cells), monocytes, CD8+ T cells, M1 macrophages, M2 macrophages, and CD4+ memory-activated T cells were the most excellent in Immunity_H, and the total survival rate was higher in the Immunity_L. Through analysis, IRF4 (MUM1) was identified by us as immunotherapeutic target and a potential prognostic marker for DLBCL, which was made sure by using molecular biology experimentations. To conclude, immunosignature made a connection between DLBCL subtypes playing a position in DLBCL prognostic stratification. Immunocharacteristics-related DLBCL subtypes\u2019 construction predicts expected patient results and supplies conceivable immunotherapy candida.<\/jats:p>","DOI":"10.1155\/2022\/9544827","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T03:05:12Z","timestamp":1660100712000},"page":"1-16","source":"Crossref","is-referenced-by-count":6,"title":["Immunogenomic-Based Analysis of Hierarchical Clustering of Diffuse Large Cell Lymphoma"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0548-1176","authenticated-orcid":true,"given":"Longhao","family":"Wang","sequence":"first","affiliation":[{"name":"Internet Medical and System Applications of National Engineering Laboratory, The First Affiliated Hospital of Zhengzhou University, China"},{"name":"Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yuan","sequence":"additional","affiliation":[{"name":"People\u2019s Hospital of Henan University of Chinese Medicine, China"},{"name":"People\u2019s Hospital of Zhengzhou, Zhengzhou, 450052 Henan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifeng","family":"Li","sequence":"additional","affiliation":[{"name":"Internet Medical and System Applications of National Engineering Laboratory, The First Affiliated Hospital of Zhengzhou University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhibo","family":"Shen","sequence":"additional","affiliation":[{"name":"Internet Medical and System Applications of National Engineering Laboratory, The First Affiliated Hospital of Zhengzhou University, China"},{"name":"Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qishun","family":"Geng","sequence":"additional","affiliation":[{"name":"Internet Medical and System Applications of National Engineering Laboratory, The First Affiliated Hospital of Zhengzhou University, China"},{"name":"Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Internet Medical and System Applications of National Engineering Laboratory, The First Affiliated Hospital of Zhengzhou University, China"},{"name":"Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1168-8384","authenticated-orcid":true,"given":"Jie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Internet Medical and System 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