{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:27:26Z","timestamp":1767166046844,"version":"build-2238731810"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":259,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jouf University","award":["DSR2022-RG-0155"],"award-info":[{"award-number":["DSR2022-RG-0155"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioinorganic Chemistry and Applications"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    In this study, cells from human Chronic Myelogenous Leukemia (K562) were cultivated with CuO\u2010TiO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010Chitosan\u2010Berbamine nanocomposites. We examined nanocomposites using XRD, DLS, FESEM, TEM, PL, EDAX, and FTIR spectroscopy, as well as MTT for cytotoxicity, and AO\/EtBr for apoptotic morphology assessment. The rate of apoptosis and cell cycle arrests was determined using flow cytometry. Flow cytometry was also employed to identify pro\u2010 and antiapoptotic proteins such as Bcl2, Bad, Bax, P53, and Cyt C. The FTIR spectrum revealed that the CuO\u2010TiO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010Chitosan\u2010Berbamine nanocomposites were electrostatically interlocked. The nanocomposites\u2019 XRD signals revealed a hexagonal shape. In the DLS spectrum, nanocomposites were found to have a hydrodynamic diameter. As a result of their cytotoxic action, nanocomposites displayed concentration\u2010dependent cytotoxicity. The nanocomposites, like Doxorubicin, caused cell cycle phase arrest in K562 cells. After treatment with IC\n                    <jats:sub>50<\/jats:sub>\n                    concentrations of CuO\u2010TiO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010Chitosan\u2010Berbamine nanocomposites and Doxorubicin, a substantial percentage of cells were in G2\/M stage arrest. Caspase\u20103, \u20107, \u20108, \u20109, Bax, Bad, Cyt C, and P53 expression were considerably enhanced in K562 cells, whereas Bcl2 expression was decreased, indicating that these cells may have therapeutic potential against human blood cancer\/leukemia\u2010derived disorders. As a result, the nanocomposites demonstrated outstanding anticancer potential against leukemic cells. CuO\u2010TiO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010Chitosan\u2010Berbamine, according to our findings.\n                  <\/jats:p>","DOI":"10.1155\/2022\/9602725","type":"journal-article","created":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T13:52:16Z","timestamp":1663422736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["[Retracted] CuO\u2010TiO2\u2010Chitosan\u2010Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL\u20102 in the Human K562 Cancer Cell Line"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6219-5312","authenticated-orcid":false,"given":"Abozer Y.","family":"Elderdery","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Badr","family":"Alzahrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siddiqa M. 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