{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:18:14Z","timestamp":1740140294458,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hunan Administration of Traditional Chinese Medicine","award":["2021057","2020JJ5436","kq2107014"],"award-info":[{"award-number":["2021057","2020JJ5436","kq2107014"]}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021057","2020JJ5436","kq2107014"],"award-info":[{"award-number":["2021057","2020JJ5436","kq2107014"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018579","name":"Training Program for Excellent Young Innovators of Changsha","doi-asserted-by":"publisher","award":["2021057","2020JJ5436","kq2107014"],"award-info":[{"award-number":["2021057","2020JJ5436","kq2107014"]}],"id":[{"id":"10.13039\/501100018579","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,8,11]]},"abstract":"<jats:p>Objective. To observe the protective effect of gynostemma glycosides on retinal ganglion cells in rats with chronically high intraocular pressure. Materials and Methods. A total of 60 rats were randomly divided into group A (the blank group, 10 rats) and chronic high IOP model group (50 rats). The IOP model group (IOP above 22\u2009mmHg) was then randomly divided into an additional 5 groups (10 rats per group): group B (negative control group) treated with normal saline; group C treated with gynostemma glycosides 25\u2009mg\/(kg-d); group D treated with gynostemma glycosides 50\u2009mg\/(kg-d); group E treated with gynostemma glycosides 100\u2009mg\/(kg-d); and group F (positive control group) treated with VitB1 and VitB12. The eyes of each rat were monitored from day 1 to 14 (D1\u2013D14). On day 14, rats were euthanized, after which retinal tissue and optic nerve were examined using real-time PCR, western blot, HE staining, LFB staining, and TUNEL assay. Results. Groups A, C, D, E, and F had significantly lower expression of CD11b, GFAP, Brn3\u03b1, and more TUNEL cells than in group B (all <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). Moreover, the relative expression of STAT3 mRNA and JAK2 (mRNA and protein) in groups A, C, D, E, and F was significantly lower than in group B (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>), while in group E, the expression was lower than in group D (<jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>P<\/e:mi>\n                        <e:mo>&lt;<\/e:mo>\n                        <e:mn>0.05<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>). Conclusion. Gynostemma glycosides protect retinal ganglion cells in rats with chronically high intraocular pressure possibly associated with the STAT3\/JAK2 signaling pathway.<\/jats:p>","DOI":"10.1155\/2022\/9963754","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T20:35:10Z","timestamp":1660250110000},"page":"1-9","source":"Crossref","is-referenced-by-count":0,"title":["Gynostemma Glycosides Protect Retinal Ganglion Cells in Rats with Chronic High Intraocular Pressure by Regulating the STAT3\/JAK2 Signaling Pathway and Inhibiting Astrocyte and Microglia Activation"],"prefix":"10.1155","volume":"2022","author":[{"given":"Xian-jing","family":"Wang","sequence":"first","affiliation":[{"name":"Hunan University of Chinese Medicine, Changsha, China"},{"name":"Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Hu","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine, Changsha, China"},{"name":"Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong-ying","family":"Huang","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine, Changsha, China"},{"name":"Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-hua","family":"Peng","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine, Changsha, China"},{"name":"Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2614-4531","authenticated-orcid":true,"given":"Juan","family":"Yu","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Changsha, China"},{"name":"The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"5","key":"1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1136\/bjophthalmol-2011-300539","article-title":"Global estimates of visual impairment 2010","volume":"96","author":"P. 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