{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T05:58:42Z","timestamp":1781330322446,"version":"3.54.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":320,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Imatinib is a classical targeted drug to treat chronic myeloid leukemia (CML). However, it shows cardiotoxicity, which limits its clinical application. Long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) shows proapoptotic properties in human cells. This study is performed to investigate whether targeting MEG3 can attenuate imatinib\u2010mediated cardiotoxicity to cardiomyocytes. In this work, H9c2 cells were divided into four groups: control group, hypoxia group, hypoxia\u2009+\u2009imatinib, and hypoxia\u2009+\u2009imatinib\u2009+\u2009MEG3 knockdown group. MEG3 and microRNA\u2010129\u20105p (miR\u2010129\u20105p) expression levels were detected by the quantitative real\u2010time PCR (qRT\u2010PCR). The viability and apoptosis of H9c2 cells were then evaluated by cell counting kit\u20108 (CCK\u20108), flow cytometry, and TUNEL assays. The targeting relationships between MEG3 and miR\u2010129\u20105p, between miR\u2010129\u20105p and high\u2010mobility group box 1 (HMBG1), were validated by dual\u2010luciferase reporter assay and RNA Immunoprecipitation (RIP) assay. The protein expression level of HMGB1 was detected by western blot. It was revealed that, Imatinib\u2010inhibited cell viability and aggravated the apoptosis of H9c2 cells cultured in hypoxic condition, and MEG3 knockdown significantly counteracted this effect. MiR\u2010129\u20105p was a downstream target of MEG3 and it directly targeted HMGB1, and knockdown of MEG3 inhibited HMGB1 expression in H9c2 cells. In conclusion, targeting MEG3 ameliorates imatinib\u2010induced injury of cardiomyocytes via regulating miR\u2010129\u20105p\/HMGB1 axis.<\/jats:p>","DOI":"10.1155\/2023\/1108280","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T21:05:09Z","timestamp":1700255109000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Depletion of lncRNA MEG3 Ameliorates Imatinib\u2010Induced Injury of Cardiomyocytes via Regulating miR\u2010129\u20105p\/HMGB1 Axis"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0245-3295","authenticated-orcid":false,"given":"Peng","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5487-6565","authenticated-orcid":false,"given":"Jinjian","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2183-2087","authenticated-orcid":false,"given":"Huagang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1163-7405","authenticated-orcid":false,"given":"Shenglan","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-0582","authenticated-orcid":false,"given":"Kai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2178-4441","authenticated-orcid":false,"given":"Hao","family":"Ming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,17]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd10090357"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737140.2016.1151356"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/02841860903229124"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152510790796192"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2020.05.077"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.302521"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.303836"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2021.08.006"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02280-y"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000906"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky722"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41434-018-0045-4"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20190210"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmy133"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000741"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11886-023-01845-2"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-13203-3"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23042282"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2021.108866"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15720"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201909_18881"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.21037\/jtd-19-2472"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11656"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27486"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.55976\/dt.22023116113-15"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.3109\/10717544.2012.738436"},{"key":"e_1_2_8_27_2","first-page":"1886","article-title":"MiR-129-5p-mediated beclin-1 suppression inhibits endothelial cell autophagy in atherosclerosis","volume":"8","author":"Geng Z.","year":"2016","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.3200"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S336626"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2021.3163"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22850"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.10.045"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.035"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvt275"}],"updated-by":[{"DOI":"10.1155\/ancp\/9761979","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000}}],"container-title":["Analytical Cellular Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2023\/1108280.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2023\/1108280.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2023\/1108280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T18:43:17Z","timestamp":1733942597000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2023\/1108280"}},"subtitle":[],"editor":[{"given":"Maria Beatrice","family":"Morelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1155\/2023\/1108280"],"URL":"https:\/\/doi.org\/10.1155\/2023\/1108280","archive":["Portico"],"relation":{},"ISSN":["2210-7177","2210-7185"],"issn-type":[{"value":"2210-7177","type":"print"},{"value":"2210-7185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"2023-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1108280"}}