{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:55:41Z","timestamp":1792090541890,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":275,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Early reperfusion into the myocardium after ischemia causes myocardial ischemia\u2013reperfusion (I\/R) injury and ferroptosis was involved. Ischemia activates the expression of a series of oxidative stress genes and their downstream regulatory genes, including ferroptosis\u2010related genes such as nuclear factor E2\u2010related factor 2 (Nrf2), glutathione peroxidase 4 (GPX4), and SLC7A11. This study adopted primary cardiomyocytes and I\/R in rats to evaluate the ferroptosis and changing of Nrf2\u2010SLC7A11\/heme oxygenase\u20101 (HO\u20101) <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. Online analysis tools were used to predict the possible target Kelch\u2010like ECH\u2010associated protein 1 (Keap1) of miR\u2010432\u20105p. The mimic of miR\u2010432\u20105p plasmid was constructed to verify the effect of miR\u2010432\u20105p on ferroptosis. We found that hypoxia\/reoxygenation (H\/R) in cardiomyocytes and I\/R in rats induced lipid peroxidation and ferroptosis in cardiomyocytes. The activation of the Nrf2\u2010SLC7A11\/HO\u20101 pathway protects cardiomyocytes from ferroptosis. Downregulation of miR\u2010432\u20105p has been confirmed in H\/R cardiomyocytes (<jats:italic>in vitro<\/jats:italic>) and cardiomyocytes in myocardial infarction rats (<jats:italic>in vivo<\/jats:italic>). Upregulation of miR\u2010432\u20105p inhibited ferroptosis of cardiomyocytes induced by RAS\u2010selective lethal 3 (RSL3), an inhibitor of GPX4 and ferroptosis inducer through decreasing the binding protein of Nrf2, Keap1, which was confirmed by bioinformatics and mutation assay. Knockdown Nrf2 attenuates the protection effect of miR\u2010432\u20105p on H\/R cardiomyocytes. Intravenous delivery of liposome carriers of miR\u2010432\u20105p remarkably ameliorated cardiomyocyte impairment in the I\/R animal model. In conclusion, miR\u2010432\u20105p inhibits the ferroptosis in cardiomyocytes induced by H\/R by activating Nrf2\/SLC7A11 axis by degrading Keap1 and is a potential drug target for clinical myocardial infarction treatment.<\/jats:p>","DOI":"10.1155\/2023\/1293200","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T22:20:08Z","timestamp":1696371608000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["miR\u2010432\u20105p Inhibits the Ferroptosis in Cardiomyocytes Induced by Hypoxia\/Reoxygenation via Activating Nrf2\/SLC7A11 Axis by Degrading Keap1"],"prefix":"10.1155","volume":"2023","author":[{"given":"Wei","family":"Geng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiumei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinshun","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7715-9592","authenticated-orcid":false,"given":"Xiang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3882-2446","authenticated-orcid":false,"given":"Yunfa","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,3]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00925-z"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2022.11357"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9060620"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202100882R"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.008024"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111939"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.799499"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934612"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.00267"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4505513"},{"key":"e_1_2_10_11_2","first-page":"10315","article-title":"Fraxetin attenuates ferroptosis in myocardial infarction via AKT\/Nrf2\/HO-1 signaling","volume":"13","author":"Xu Y.","year":"2021","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2223-0"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-021-00579-w"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04054-3"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.09.014"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2011.09.002"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103378"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-26780-3_8"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-022-10245-0"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13286"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S323437"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-17-0232"},{"key":"e_1_2_10_24_2","series-title":"Methods in Molecular Biology","first-page":"321","volume-title":"Cardiac Gene Expression","author":"Maass A. 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