{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:44:24Z","timestamp":1790117064980,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Danish Dental Association"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Dentistry"],"published-print":{"date-parts":[[2023,4,27]]},"abstract":"<jats:p>Aim. There is need of an objective \u201cstandard procedure\u201d that is reliable and clinically applicable for estimating oral neutrophil content in relation to oral diseases. Methods. Forty-one patients with suspected oral candidosis (OC) and nine healthy controls with no oral mucosal disease were flushing with 10\u2009ml mouth rinse (MR) (sterile phosphate-buffered saline) for 1\u2009min. Aliquots were stored on different conditions to explore stability, storage, and fixation conditions for analysis by flow cytometry. Results. The optimal storage and fixation condition for MR was by fixation 1\u2009:\u20091 in 10% formalin and stored at 5\u00b0C. This procedure yielded stable results up to 7 days after collection. The ability of the optimized method to relate oral neutrophils to inflammation was demonstrated by the significantly higher number of neutrophils in patients with primary OC (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>p<\/a:mi>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>0.0334<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>) compared to healthy controls. Conclusion. This method is rapid, reliable, and clinically applicable for establishing the content of oral neutrophils. We demonstrate increased density of oral neutrophils in the MR of patients with OC. The potential of the method is to be \u201cthe standard procedure\u201d for investigation of the oral inflammation in patients with oral diseases as it is noninvasive and provides high stability, clinical relevance, and minimal handling.<\/jats:p>","DOI":"10.1155\/2023\/1308326","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T04:44:52Z","timestamp":1682657092000},"page":"1-6","source":"Crossref","is-referenced-by-count":4,"title":["\u201cThe Standard Procedure\u201d for Investigation of Oral Neutrophils in Oral Diseases"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1648-7186","authenticated-orcid":true,"given":"Peter \u00d8strup","family":"Jensen","sequence":"first","affiliation":[{"name":"Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark"},{"name":"Department of Clinical Microbiology, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark"},{"name":"Center for Rheumatology and Spine Diseases, Institute for Inflammation Research, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pernille Dukanovic","family":"Rikvold","sequence":"additional","affiliation":[{"name":"Department of Odontology, Oral Pathology & Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine R\u00f8n","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Odontology, Oral Pathology & Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette Rose","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Department of Odontology, Oral Pathology & Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0892-4972","authenticated-orcid":true,"given":"Camilla","family":"Kragelund","sequence":"additional","affiliation":[{"name":"Department of Odontology, Oral Pathology & Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1994.tb00086.x"},{"key":"2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-3-319-64113-3_3","article-title":"Candida and candidiasis","volume-title":"Current Progress in Medical Mycology","author":"L. 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