{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:33:14Z","timestamp":1790843594057,"version":"4.1.0"},"reference-count":92,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:00:00Z","timestamp":1687219200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NV18-09-00493"],"award-info":[{"award-number":["NV18-09-00493"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NV18-05-00394"],"award-info":[{"award-number":["NV18-05-00394"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["61388971"],"award-info":[{"award-number":["61388971"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["LQ200202105"],"award-info":[{"award-number":["LQ200202105"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021831","name":"Institute of Microbiology, Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["NV18-09-00493"],"award-info":[{"award-number":["NV18-09-00493"]}],"id":[{"id":"10.13039\/501100021831","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021831","name":"Institute of Microbiology, Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["NV18-05-00394"],"award-info":[{"award-number":["NV18-05-00394"]}],"id":[{"id":"10.13039\/501100021831","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021831","name":"Institute of Microbiology, Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["61388971"],"award-info":[{"award-number":["61388971"]}],"id":[{"id":"10.13039\/501100021831","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021831","name":"Institute of Microbiology, Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["LQ200202105"],"award-info":[{"award-number":["LQ200202105"]}],"id":[{"id":"10.13039\/501100021831","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Czech Academy of Sciences","award":["NV18-09-00493"],"award-info":[{"award-number":["NV18-09-00493"]}]},{"name":"Czech Academy of Sciences","award":["NV18-05-00394"],"award-info":[{"award-number":["NV18-05-00394"]}]},{"name":"Czech Academy of Sciences","award":["61388971"],"award-info":[{"award-number":["61388971"]}]},{"name":"Czech Academy of Sciences","award":["LQ200202105"],"award-info":[{"award-number":["LQ200202105"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2023,6,20]]},"abstract":"<jats:p>Background. Tumor necrosis factor-alpha (TNF-\u03b1) agonists revolutionized therapeutic algorithms in inflammatory bowel disease (IBD) management. However, approximately every third IBD patient does not respond to this therapy in the long term, which delays efficient control of the intestinal inflammation. Methods. We analyzed the power of serum biomarkers to predict the failure of anti-TNF-\u03b1. We collected serum of 38 IBD patients at therapy prescription and 38 weeks later and analyzed them with relation to therapy response (no-, partial-, and full response). We used enzyme-linked immunosorbent assay to quantify 16 biomarkers related to gut barrier (intestinal fatty acid-binding protein, liver fatty acid-binding protein, trefoil factor 3, and interleukin (IL)-33), microbial translocation, immune system regulation (TNF-\u03b1, CD14, lipopolysaccharide-binding protein, mannan-binding lectin, IL-18, transforming growth factor-\u03b21 (TGF-\u03b21), osteoprotegerin (OPG), insulin-like growth factor 2 (IGF-2), endocrine-gland-derived vascular endothelial growth factor), and matrix metalloproteinase system (MMP-9, MMP-14, and tissue inhibitors of metalloproteinase-1). Results. We found that future full-responders have different biomarker profiles than non-responders, while partial-responders cannot be distinguished from either group. When future non-responders were compared to responders, their baseline contained significantly more TGF-\u03b21, less CD14, and increased level of MMP-9, and concentration of these factors could predict non-responders with high accuracy (AUC\u2009=\u20090.938). Interestingly, during the 38 weeks, levels of MMP-9 decreased in all patients, irrespective of the outcome, while OPG, IGF-2, and TGF-\u03b21 were higher in non-responders compared to full-responders both at the beginning and the end of the treatment. Conclusions. The TGF-\u03b21 and CD14 can distinguish non-responders from responders. The changes in biomarker dynamics during the therapy suggest that growth factors (such as OPG, IGF-2, and TGF-\u03b2) are not markedly influenced by the treatment and that anti-TNF-\u03b1 therapy decreases MMP-9 without influencing the treatment outcome.<\/jats:p>","DOI":"10.1155\/2023\/1535484","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T22:35:43Z","timestamp":1687300543000},"page":"1-16","source":"Crossref","is-referenced-by-count":6,"title":["Serum TGF-\u03b21 and CD14 Predicts Response to Anti-TNF-\u03b1 Therapy in IBD"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6950-1813","authenticated-orcid":true,"given":"Stepan","family":"Coufal","sequence":"first","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miloslav","family":"Kverka","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Kreisinger","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Evolutionary Biology, Faculty of Science, Department of Zoology, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Thon","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Rob","sequence":"additional","affiliation":[{"name":"Second Faculty of Medicine, University Hospital Bulovka, Dermatovenerology Department, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kolar","sequence":"additional","affiliation":[{"name":"ISCARE a.s., IBD Clinical and Research Centre, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Reiss","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagmar","family":"Schierova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klara","family":"Kostovcikova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radka","family":"Roubalova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Bajer","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"},{"name":"Department of Gastroenterology and Hepatology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Jackova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Mihula","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Drastich","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Tresnak Hercogova","sequence":"additional","affiliation":[{"name":"Second Faculty of Medicine, University Hospital Bulovka, Dermatovenerology Department, Charles University, Prague, Czech Republic"},{"name":"Dermatology Prof. Hercogova, Center for Biological Therapy, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Novakova","sequence":"additional","affiliation":[{"name":"Second Faculty of Medicine, University Hospital Bulovka, Dermatovenerology Department, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Vasatko","sequence":"additional","affiliation":[{"name":"ISCARE a.s., IBD Clinical and Research Centre, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Lukas","sequence":"additional","affiliation":[{"name":"ISCARE a.s., IBD Clinical and Research Centre, Prague, Czech Republic"},{"name":"Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Tlaskalova-Hogenova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6695-8188","authenticated-orcid":true,"given":"Zuzana","family":"Jiraskova Zakostelska","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)32448-0"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/jcag\/gwaa028"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI155786"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08512-4"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2011.07.011"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2008.03.014"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2011.73"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2010.07.022"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6284987"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.814429"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18091973"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13139"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000225"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1080\/gas.37.7.818.824"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2011.04987.x"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjy156"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.12324"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0b013e31829cf401"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.221127"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.178665"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/tpj.2014.19"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1038\/tpj.2016.84"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098815"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13002"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070719"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3074313"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2022.155890"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(80)92767-1"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198712243172603"},{"issue":"2","key":"30","first-page":"233","article-title":"Anti-TNF therapy in inflammatory bowel diseases: a huge review","volume":"56","author":"L. Peyrin-Biroulet","year":"2010","journal-title":"Minerva Gastroenterologica e Dietologica"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2015.06.001"},{"key":"32","article-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2020"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082099299"},{"key":"34","doi-asserted-by":"crossref","DOI":"10.1007\/978-0-387-21706-2","volume-title":"Modern Applied Statistics with S","author":"W. N. Venables","year":"2002","edition":"4th"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti623"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v025.i01"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00517"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1177\/2040622319899297"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.601842"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000003417"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5727312"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210797"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/s0009-9120(03)00096-1"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.13.4680-4690.2000"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.15403\/jgld-177"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01091"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2019.02.006"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.581445"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-010-0245-1"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301785"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1172\/JCI88625"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912678107"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1210\/jendso\/bvz039"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6942-6950.2001"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31823a895a"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.7.4470"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3772-3781.2001"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(96)80908-X"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1038\/386506a0"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.5.1733"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6363784"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-1065-2_42"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.208348"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2012.03.008"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2011.594087"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.3.1627"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6665"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.10.072"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2019.4156"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0205061"},{"key":"73","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20081887"},{"key":"74","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.12.016"},{"key":"75","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02643.x"},{"key":"76","doi-asserted-by":"publisher","DOI":"10.1159\/000087190"},{"key":"77","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-017-1350-1"},{"key":"78","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2000.00861.x"},{"key":"79","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02250.x"},{"key":"80","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90314-O"},{"key":"81","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0b013e3182a8452e"},{"key":"82","doi-asserted-by":"publisher","DOI":"10.1159\/000336995"},{"key":"83","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-011-9420-9"},{"key":"84","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20419"},{"key":"85","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0249544"},{"key":"86","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-017-1510-z"},{"key":"87","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2017.05.010"},{"key":"88","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.06.019"},{"key":"89","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2003.01574.x"},{"key":"90","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjaa139"},{"key":"91","doi-asserted-by":"publisher","DOI":"10.1016\/S0399-8320(05)80718-3"},{"key":"92","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2014.01.010"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2023\/1535484.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2023\/1535484.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2023\/1535484.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T22:35:49Z","timestamp":1687300549000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2023\/1535484\/"}},"subtitle":[],"editor":[{"given":"Weirong","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,6,20]]},"references-count":92,"alternative-id":["1535484","1535484"],"URL":"https:\/\/doi.org\/10.1155\/2023\/1535484","relation":{},"ISSN":["2314-7156","2314-8861"],"issn-type":[{"value":"2314-7156","type":"electronic"},{"value":"2314-8861","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6,20]]}}}