{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:11:57Z","timestamp":1790885517626,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":93,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Acetaminophen has always been at the center of attention as a non\u2010steroidal anti\u2010inflammatory drug, which is generally associated with the serious side effects on liver and the hematological parameters. This study aimed to compare the effect of N\u2010acetyl cysteine (NAC) and thyme extract on rat models of acetaminophen\u2010induced toxicity. The present experimental study was conducted on 48 Wistar rats randomized into six groups, including the control group (no treatment); the Ac group (470 mg\/kg of acetaminophen); the Ac\u2009+\u2009100Ex, Ac\u2009+\u2009200Ex, and Ac\u2009+\u2009400Ex groups (acetaminophen\u2009+\u2009thyme extract at doses of 100, 200, 400\u2009mg\/kg); and Ac\u2009+\u2009NA group (acetaminophen\u2009+\u2009NAC). After weighing, a blood sample was taken from heart at the end of the period. The measured parameters were hematological, liver biochemical, and oxidative stress profiles. A part of the liver tissue was also fixed for the pathological examinations. The bone marrow was aspirated to check for cellular changes as well. The lowest mean of the final weight and liver weight to body weight ratio was observed in the Ac group. Weight loss was compensated in Ac\u2009+\u2009NA and Ac\u2009+\u2009200Ex groups (<jats:italic>P<\/jats:italic> = 0.035). White blood cell (WBC), red blood cell (RBC), Hemoglobin (Hgb), and Hematocrit (HCT) in Ac and Ac\u2009+\u2009400Ex groups showed significant differences from those of the other test groups (<jats:italic>P<\/jats:italic> &lt; 0.001). Aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) enzymes in Ac\u2009+\u2009200Ex and Ac\u2009+\u2009NA groups showed a significant decrease compared to those of the other treatment groups (<jats:italic>P<\/jats:italic> = 0.043). Total antioxidant capacity (TAC) and glutathione peroxidase (GPx) had the lowest levels in Ac and Ac\u2009+\u2009400Ex groups, while malondialdehyde (MDA) had the highest content. In this regard, the liver histopathological indices (necrosis, hyperemia, and hemorrhage) in the Ac\u2009+\u2009200Ex and Ac\u2009+\u2009NA groups reached their lowest grades in the treatment groups. The mean number of erythroid and myeloid cells in the Ac group reached the lowest (17.40 \u00b1 3.48). The microscopic appearance of the bone marrow cells was different from normocytosis in the control group to hypocytosis in the Ac and Ac\u2009+\u2009400Ex groups. Thymol, as an effective ingredient in thyme extract at a dose of 200\u2009mg\/kg compared to NAC, had a unique effect on reducing bone marrow and liver cell\u2010tissue changes due to the acetaminophen toxicity.<\/jats:p>","DOI":"10.1155\/2023\/1714884","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T20:07:42Z","timestamp":1680638862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparative Effect of the Active Substance of Thyme with N\u2010Acetyl Cysteine on Hematological Parameters and Histopathological Changes of Bone Marrow and Liver in Rat Models of Acetaminophen Toxicity"],"prefix":"10.1155","volume":"2023","author":[{"given":"Zahra","family":"Mokhtari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3460-8594","authenticated-orcid":false,"given":"Mahdieh","family":"Raeeszadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loghman","family":"Akradi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/23328940.2021.1886392"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apha.2018.12.004"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/03602532.2011.602688"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13878"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-659516"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s43066-022-00206-y"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.17582\/journal.aavs\/2018\/6.9.366.371"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.12.023"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/1828051X.2021.1965923"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738053"},{"key":"e_1_2_9_11_2","article-title":"The multifaceted therapeutic role of N-Acetylcysteine (NAC) in disorders characterized by oxidative stress","volume":"19","author":"Raghu G.","year":"2021","journal-title":"Current Neuropharmacology"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.18869\/acadpub.hrjbaq.1.3.133"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022020000501444"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40360-020-00469-4"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.5487\/TR.2019.35.3.225"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(84)90277-1"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2691577"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1515\/tjb-2018-0258"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1063\/5.0052627"},{"key":"e_1_2_9_20_2","first-page":"593","article-title":"Dose-response changes of Brassica oleracea var. italica hydroalcholic extract in the control of oxidative stress by induction of diazinon on the cells of testicular tissue in male adult rat","volume":"16","author":"Qaderi Forough M.","year":"2017","journal-title":"Journal of Rafsanjan University of Medical Sciences"},{"key":"e_1_2_9_21_2","first-page":"283","article-title":"Assessment the effect of saffron ethanolic extract (Crocus sativus L.) on oxidative damages in aged male rat liver","volume":"8","author":"Samarghandian S.","year":"2016","journal-title":"Der Pharmacia Lettre"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2021.1895826"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-021-02616-2"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2051-1"},{"key":"e_1_2_9_25_2","article-title":"Alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) Activities in selected tissues of rats fed on processed atlantic horse mackerel (Trachurus trachurus)","volume":"6","author":"Adeyemi O. 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