{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:58:51Z","timestamp":1776016731179,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK107474"],"award-info":[{"award-number":["DK107474"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:p>Salmonella Typhimurium infection of the gastrointestinal tract leads to damage that compromises the integrity of the intestinal epithelium and results in enterocolitis and inflammation. Salmonella infection promotes the expression of inflammasome NLRP3, leading to activation and release of proinflammatory cytokines such as IL-1\u03b2, and the infected host often displays altered nutrient levels. To date, the effect of Salmonella infection and proinflammatory cytokine IL-1\u03b2 on the intestinal uptake of ascorbic acid (AA) is unknown. Our results revealed a marked decrease in the rate of AA uptake in mouse jejunum infected with Salmonella wild type (WT). However, the nonpathogenic mutant (\u0394 invA \u0394 spiB) strain did not affect AA uptake. The decrease in AA uptake due to Salmonella WT infection is accompanied by significantly lower expression of mouse (m)SVCT1 protein, mRNA, and hnRNA levels. NLRP3 and IL-1\u03b2 expression levels were markedly increased in Salmonella-infected mouse jejunum. IL-1\u03b2-exposed Caco-2 cells displayed marked inhibition in AA uptake and significantly decreased hSVCT1 expression at both protein and mRNA levels. Furthermore, the activity of the SLC23A1 promoter was significantly inhibited by IL-1\u03b2 exposure. In addition, GRHPR (a known SVCT1 interactor) protein and mRNA expression levels were significantly reduced in Salmonella-infected mouse jejunum. These results indicate that Salmonella infection inhibits AA absorption in mouse jejunum and IL-1\u03b2-exposed Caco-2 cells. The observed inhibitory effect may partially be mediated through transcriptional mechanisms.<\/jats:p>","DOI":"10.1155\/2023\/2629262","type":"journal-article","created":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T14:50:14Z","timestamp":1673967014000},"page":"1-9","source":"Crossref","is-referenced-by-count":7,"title":["Salmonella Typhimurium Infection Reduces the Ascorbic Acid Uptake in the Intestine"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5576-8509","authenticated-orcid":true,"given":"Trevor","family":"Teafatiller","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology, University of California, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep B.","family":"Subramanya","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box-15551, Al Ain, UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils","family":"Lambrecht","sequence":"additional","affiliation":[{"name":"Department of Veteran Affairs Medical Center, Long Beach, CA 90822, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1159-3916","authenticated-orcid":true,"given":"Veedamali S.","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology, University of California, Irvine, CA 92697, USA"},{"name":"Department of Veteran Affairs Medical Center, Long Beach, CA 90822, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","volume-title":"Vitamin C in Health and Disease","author":"L. Packer","year":"1997"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/69.6.1086"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.endonu.2012.09.006"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.160.7.931"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.08699.x"},{"key":"6","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1302\/0301-620X.89B3.18007","article-title":"Effect of vitamin C on fracture healing in elderly osteogenic disorder Shionogi rats","volume":"89","author":"T. Alcantara-Martos","year":"2007","journal-title":"Journal of Bone and Joint Surgery. British Volume (London)"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.79.2.158"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.27016"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3109\/10715769509147535"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2008.01.007"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8553(05)70010-8"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)94362-H"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1093\/crocol\/otab009"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2019.07.054"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-019-0532-9"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2016.11.036"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01393-9"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1272"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1929"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(99)00182-0"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-007-0555-7"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00369.2016"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205119200"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.06.069"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400876200"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-50772-2"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2000.0750"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1086\/649541"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)61752-2"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002933"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100007"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-197912000-00001"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1080\/00365540410026068"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.1.1-12.2003"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01479-4"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22179355"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02495"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1038\/nm1743"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-42-5-348"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1993.1243"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.3.1134-1137.1995"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1993.1021"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1204721"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2016.11.009"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-014-0030-7"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601416"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100257"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1317643111"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00112.2015"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01445.x"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00071.2018"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00295.2018"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1111\/apt.15770"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2018.09.034"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.629-640.2003"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2839-2858.2003"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.2144\/19962003470"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-020-06389-x"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.10.010"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.08.008"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63555-9"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-0007-8"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00090.2013"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2023\/2629262.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2023\/2629262.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2023\/2629262.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T14:50:20Z","timestamp":1673967020000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2023\/2629262\/"}},"subtitle":[],"editor":[{"given":"Fumio","family":"Tsuji","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,1,17]]},"references-count":63,"alternative-id":["2629262","2629262"],"URL":"https:\/\/doi.org\/10.1155\/2023\/2629262","relation":{},"ISSN":["1466-1861","0962-9351"],"issn-type":[{"value":"1466-1861","type":"electronic"},{"value":"0962-9351","type":"print"}],"subject":[],"published":{"date-parts":[[2023,1,17]]}}}