{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T21:58:30Z","timestamp":1759960710065,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T00:00:00Z","timestamp":1674432000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003500","name":"Universit\u00e0 degli Studi di Padova","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003500","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>The present study was carried out to investigate the anti\u2010inflammatory activity of a methanolic extract and fractions of <jats:italic>Uvaria comperei<\/jats:italic> stems. The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti\u2010inflammatory activity <jats:italic>in vitro<\/jats:italic>, red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while <jats:italic>in vivo<\/jats:italic> measurements of carrageenan\u2010induced paw oedema and formalin\u2010induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. <jats:italic>Uvaria comperei<\/jats:italic> crude extract (UCCE) at 250 and 500\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. The fractions 128\u2010136 (F3), 10\u201011 (F1), and 56\u201062 (F2) at 500\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL displayed a significant anti\u2010inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100\u2009mg\/kg, p.o.) produced a dose\u2010dependent inhibition of formalin\u2010induced pain of 60.2% at 50\u2009mg\/kg in the neurogenic phase (<jats:italic>p<\/jats:italic> &lt; 0.05) and 70.2% at 25\u2009mg\/kg in the inflammatory phase (<jats:italic>p<\/jats:italic> &lt; 0.05). Similarly, the time\u2010dependent increase in carrageenan\u2010induced paw circumference induced by carrageenan was inhibited by pretreatment with F2: 50% of inhibition at 25\u2009mg\/kg after 30\u2009min (<jats:italic>p<\/jats:italic> &lt; 0.05) and 96.5% inhibition at 25\u2009mg\/kg after 6\u2009h (<jats:italic>p<\/jats:italic> &lt; 0.05). In this research, the fraction F2 presented its maximum analgesic property at 50\u2009mg\/kg, while it presented the highest anti\u2010inflammatory property at 25\u2009mg\/kg. The oral lethal median dose (LD<jats:sub>50<\/jats:sub>) of F2 was determined to be greater than 2000\u2009mg\/kg; further low cytotoxicity in RAW cells was also observed. Overall, this work shows that the methanolic crude extract and fractions, mainly F2, of <jats:italic>Uvaria comperei<\/jats:italic> stem have interesting anti\u2010inflammatory properties.<\/jats:p>","DOI":"10.1155\/2023\/2754725","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T03:20:05Z","timestamp":1674530405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti\u2010inflammatory, Antinociceptive, and Toxicological Properties of <i>Uvaria comperei<\/i> Stem Crude Extract and Fractions"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1556-332X","authenticated-orcid":false,"given":"Marguerite Kamdem","family":"Simo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1771-1939","authenticated-orcid":false,"given":"Gael Tchokomeni","family":"Siwe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurice","family":"Taboula Kayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0170-9516","authenticated-orcid":false,"given":"Mersimine","family":"Mangoua Kouamo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3387-4102","authenticated-orcid":false,"given":"Darline","family":"Dize","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre Dongmo","family":"Jazet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Modeste Lambert","family":"Sameza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-364X","authenticated-orcid":false,"given":"Fabrice Boyom","family":"Fekam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5936-1789","authenticated-orcid":false,"given":"Guglielmina","family":"Froldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,23]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-29452014000500038"},{"key":"e_1_2_9_2_2","first-page":"76","article-title":"Antioxidant potential of fractions from the stem methanol extract of Uvaria comperei (Annonaceae)","volume":"7","author":"Simo M.","year":"2020","journal-title":"International Journal of Pharmacognosy"},{"key":"e_1_2_9_3_2","first-page":"1478","article-title":"Cameroonian medicinal plants belonging to Annonaceae family: radical scavenging and antifungal activities","volume":"32","author":"Simo K. 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