{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:26:59Z","timestamp":1792160819804,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["1420Y503"],"award-info":[{"award-number":["1420Y503"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["SHDC2020CR3060B"],"award-info":[{"award-number":["SHDC2020CR3060B"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["XK2020013"],"award-info":[{"award-number":["XK2020013"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["20152226"],"award-info":[{"award-number":["20152226"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Shenkang Project","award":["1420Y503"],"award-info":[{"award-number":["1420Y503"]}]},{"name":"Shanghai Shenkang Project","award":["SHDC2020CR3060B"],"award-info":[{"award-number":["SHDC2020CR3060B"]}]},{"name":"Shanghai Shenkang Project","award":["XK2020013"],"award-info":[{"award-number":["XK2020013"]}]},{"name":"Shanghai Shenkang Project","award":["20152226"],"award-info":[{"award-number":["20152226"]}]},{"name":"Industrial Support Fund of Huangpu District","award":["1420Y503"],"award-info":[{"award-number":["1420Y503"]}]},{"name":"Industrial Support Fund of Huangpu District","award":["SHDC2020CR3060B"],"award-info":[{"award-number":["SHDC2020CR3060B"]}]},{"name":"Industrial Support Fund of Huangpu District","award":["XK2020013"],"award-info":[{"award-number":["XK2020013"]}]},{"name":"Industrial Support Fund of Huangpu District","award":["20152226"],"award-info":[{"award-number":["20152226"]}]},{"DOI":"10.13039\/501100003395","name":"Shanghai Municipal Education Commission","doi-asserted-by":"publisher","award":["1420Y503"],"award-info":[{"award-number":["1420Y503"]}],"id":[{"id":"10.13039\/501100003395","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003395","name":"Shanghai Municipal Education Commission","doi-asserted-by":"publisher","award":["SHDC2020CR3060B"],"award-info":[{"award-number":["SHDC2020CR3060B"]}],"id":[{"id":"10.13039\/501100003395","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003395","name":"Shanghai Municipal Education Commission","doi-asserted-by":"publisher","award":["XK2020013"],"award-info":[{"award-number":["XK2020013"]}],"id":[{"id":"10.13039\/501100003395","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003395","name":"Shanghai Municipal Education Commission","doi-asserted-by":"publisher","award":["20152226"],"award-info":[{"award-number":["20152226"]}],"id":[{"id":"10.13039\/501100003395","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2023,1,12]]},"abstract":"<jats:p>Excessive bone resorption due to increased inflammatory factors is a common feature of inflammatory lytic bone diseases. This group of diseases is effectively treated with drugs. In recent years, many studies have reported that traditional Chinese medicine herbs have substantial effects on inflammation, osteoclast differentiation and maturation, and bone destruction. Herein, we investigated the effects of osthole (OST) on lipopolysaccharide- (LPS-) induced macrophage polarization, inflammatory responses, and osteolysis. In vitro, we used immunofluorescence and quantitative real-time polymerase chain reaction assays to confirm whether bone marrow-derived macrophages showed an increased expression of inflammatory factors, such as interleukin-6, iNOS, CCR7, and CD86, in the presence of LPS. However, we found that such expression was suppressed and that the M2 macrophage expression increased in the presence of OST. OST reduced LPS- and RANKL-induced intracellular reactive oxygen species production in the bone marrow-derived macrophages. Further, it potently suppressed osteoclast differentiation and osteoclast-specific gene expression by suppressing the P38\/MAPK and NF-\u03baB pathways. Consistent with the in vitro observations, OST greatly ameliorated LPS-induced bone resorption and modulated the ratio of macrophages at the site of osteolysis. Taken together, OST has great potential for use in the management of osteolytic diseases.<\/jats:p>","DOI":"10.1155\/2023\/2975193","type":"journal-article","created":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T23:05:10Z","timestamp":1673564710000},"page":"1-14","source":"Crossref","is-referenced-by-count":12,"title":["Osthole Inhibits M1 Macrophage Polarization and Attenuates Osteolysis in a Mouse Skull Model"],"prefix":"10.1155","volume":"2023","author":[{"given":"Feifei","family":"Wang","sequence":"first","affiliation":[{"name":"The Affiliated Tai\u2019an City Central Hospital of Qingdao University, Tai\u2019an, 271000 Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuzhou Central Hospital, Xuzhou, 221000 Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhao","family":"Wan","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Liu","sequence":"additional","affiliation":[{"name":"The Affiliated Tai\u2019an City Central Hospital of Qingdao University, Tai\u2019an, 271000 Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Tai\u2019an Second Hospital of Traditional Chinese Medicine, Tai\u2019an, 271000 Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1276-1662","authenticated-orcid":true,"given":"Xuzhuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Stomatology, Department of Oral Surgery, Ninth People\u2019s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, No. 639, Zhi Zao Ju Rd, Shanghai 200011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-3618","authenticated-orcid":true,"given":"Huanyan","family":"Liu","sequence":"additional","affiliation":[{"name":"The Affiliated Tai\u2019an City Central Hospital of Qingdao University, Tai\u2019an, 271000 Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04151206"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-021-04111-0"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/jicd.12091"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00084-17"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10111350"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01408"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12983"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2017.07.019"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00637"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3390\/nu12102999"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-014-0195-2"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24702"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.71"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.10.018"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.108934"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052431"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1159\/000328776"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802364RR"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.3109\/s10165-006-0515-1"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2008.07.002"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1177\/2042098611411012"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183693"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03235-w"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515591790"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.09.024"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-021-02512-z"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2019.01.015"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.041"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2018.07.007"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.09.034"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201801977R"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.12.018"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.7150\/thno.30206"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.10.016"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmaa098"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28699"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2023\/2975193.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2023\/2975193.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2023\/2975193.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T23:05:16Z","timestamp":1673564716000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/omcl\/2023\/2975193\/"}},"subtitle":[],"editor":[{"given":"Gaocai","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1,12]]},"references-count":37,"alternative-id":["2975193","2975193"],"URL":"https:\/\/doi.org\/10.1155\/2023\/2975193","relation":{},"ISSN":["1942-0994","1942-0900"],"issn-type":[{"value":"1942-0994","type":"electronic"},{"value":"1942-0900","type":"print"}],"subject":[],"published":{"date-parts":[[2023,1,12]]}}}