{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:08:53Z","timestamp":1787782133430,"version":"build-2784847793"},"reference-count":59,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T00:00:00Z","timestamp":1697587200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["218S462"],"award-info":[{"award-number":["218S462"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["42792"],"award-info":[{"award-number":["42792"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007504","name":"Istanbul Technical University","doi-asserted-by":"crossref","award":["218S462"],"award-info":[{"award-number":["218S462"]}],"id":[{"id":"10.13039\/501100007504","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007504","name":"Istanbul Technical University","doi-asserted-by":"crossref","award":["42792"],"award-info":[{"award-number":["42792"]}],"id":[{"id":"10.13039\/501100007504","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2023,10,18]]},"abstract":"<jats:p>Immune complexes (ICs) skew immune responses toward either a pro- or anti-inflammatory direction based on the type of stimulation. Immunoglobulin E (IgE) is associated with Th2 immune responses and known to activate innate immune cells. However, roles of antigen (Ag)-specific-IgE ICs in regulating human eosinophil responses remain elusive; therefore, this study builts upon the mechanism of which ovalbumin (Ova)-IgE ICs affects eosinophilic responses utilizing human EoL-1 cell line as a model. Eosinophils are granulocytes functioning through pattern recognition receptors (PRRs) and destructive granule contents in allergic inflammation and parasitic infections. One of the PRRs that eosinophils express is NLRC4, a member of the CARD domain containing nucleotide-binding oligomerization (NOD)-like receptor (NLR) family. Upon recognition of its specific ligand flagellin, NLRC4 inflammasome is formed and leads to the release of interleukin-1\u03b2 (IL-1\u03b2). We exhibited that Ova-IgE ICs induced the NLRC4-inflammasome components, including NLRC4, caspase-1, intracellular IL-1\u03b2, and secretion of IL-1\u03b2, as well as the granule contents MMP9, TIMP1, and TIMP2 proteins via TLR2 signaling; these responses were suppressed, when NLRC4 inflammasome got actived in the presence of ICs. Furthermore, Ova-IgE ICs induced mRNA expressions of MMP9, TIMP2, and ECP and protein expressions of MMP9 and TIMP2 in EoL-1 through Fc\u025bRII. Interestingly, TLR2 ligand and Ova-IgE ICs costimulation elevated the number of CD63+ cells, a degranulation marker, as compared to the native IgE. Collectively, our findings provide a mechanism for the impacts of Ova-IgE ICs on eosinophilic responses via NLRC4-inflammasome and may help understand eosinophil-associated diseases, including chronic eosinophilic pneumonia, eosinophilic esophagitis, eosinophilic granulomatosis, parasitic infections, allergy, and asthma.<\/jats:p>","DOI":"10.1155\/2023\/3224708","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T01:05:20Z","timestamp":1697677520000},"page":"1-15","source":"Crossref","is-referenced-by-count":5,"title":["IgE Immune Complexes Mitigate Eosinophilic Immune Responses through NLRC4 Inflammasome"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4234-9086","authenticated-orcid":true,"given":"Ece","family":"Oylumlu","sequence":"first","affiliation":[{"name":"Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul 34469, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2718-6197","authenticated-orcid":true,"given":"Goksu","family":"Uzel","sequence":"additional","affiliation":[{"name":"Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul 34469, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6648-4197","authenticated-orcid":true,"given":"Lubeyne","family":"Durmus","sequence":"additional","affiliation":[{"name":"Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul 34469, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2162-0930","authenticated-orcid":true,"given":"Ceren","family":"Ciraci","sequence":"additional","affiliation":[{"name":"Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul 34469, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-44887-1.00007-9"},{"issue":"1","key":"2","doi-asserted-by":"crossref","DOI":"10.1186\/s12931-018-0813-0","article-title":"Is IgE or eosinophils the key player in allergic asthma pathogenesis? Are we asking the right question?","volume":"19","author":"A. Matucci","year":"2018","journal-title":"Respiratory Research"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.11.017"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nri914"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.5.2667"},{"issue":"1","key":"6","first-page":"56","article-title":"Extracellular release of peroxidase from eosinophils by interaction with immune complexes","volume":"28","author":"T. Takenaka","year":"1977","journal-title":"Clinical & Experimental Immunology"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3390\/antib8010019"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/ni811"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.331967"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.776476"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2795"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29664-4"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1159\/000445215"},{"issue":"47","key":"14","doi-asserted-by":"crossref","first-page":"18478","DOI":"10.1073\/pnas.0804547105","article-title":"Eosinophil granules function extracellularly as receptor-mediated secretory organelles","volume":"105","author":"J. S. Neves","year":"2008","journal-title":"The Proceedings of the National Academy of Sciences USA"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2012.03556.x"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2012.07.007"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0411183"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101405"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.007"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.148"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201201771"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00309"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.4110\/in.2021.21.e42"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.3109\/10428199209053629"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.906450"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.3892\/or.2017.5383"},{"issue":"5","key":"27","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftu003","article-title":"Resistance of MMP9 and TIMP1 to endotoxin tolerance","volume":"73","author":"M. Muthukuru","year":"2015","journal-title":"Pathogens and Disease"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-147"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.4110\/in.2015.15.6.313"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.11.012"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1421795"},{"issue":"3","key":"32","doi-asserted-by":"crossref","first-page":"e0151252","DOI":"10.1371\/journal.pone.0151252","article-title":"Immune complexes indirectly suppress the generation of Th17 responses in vivo","volume":"11","author":"C. Ciraci","year":"2016","journal-title":"PLoS One"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400628"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2019.11.002"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3A1015-480R"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000070112.80711.3D"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.01.011"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00146510"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2015-0668"},{"issue":"2","key":"40","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s00726-010-0689-x","article-title":"Physiology and pathophysiology of matrix metalloproteases","volume":"41","author":"T. Klein","year":"2011","journal-title":"Amino Acids"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1136\/thx.54.7.590"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.6.9803014"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4761507"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02559-5"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20141388"},{"issue":"10","key":"46","doi-asserted-by":"crossref","first-page":"5228","DOI":"10.4049\/jimmunol.163.10.5228","article-title":"Primary peripheral blood eosinophils rapidly degrade transfected granulocyte-macrophage colony-stimulating factor mRNA","volume":"163","author":"S. Esnault","year":"1999","journal-title":"Journal of Immunology"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.132"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2017.07.003"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07457.x"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(06)93002-8"},{"issue":"2","key":"51","first-page":"341","article-title":"Omalizumab (Xolair) for treatment of asthma","volume":"71","author":"L. Davydov","year":"2005","journal-title":"American Family Physician"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035994"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v99.11.4039"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1159\/000475298"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.08.055"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20142182"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1021\/bi981456k"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01768.x"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.5MR0118-028R"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2023\/3224708.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2023\/3224708.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2023\/3224708.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T01:05:26Z","timestamp":1697677526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2023\/3224708\/"}},"subtitle":[],"editor":[{"given":"Yasumasa","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,10,18]]},"references-count":59,"alternative-id":["3224708","3224708"],"URL":"https:\/\/doi.org\/10.1155\/2023\/3224708","relation":{},"ISSN":["1466-1861","0962-9351"],"issn-type":[{"value":"1466-1861","type":"electronic"},{"value":"0962-9351","type":"print"}],"subject":[],"published":{"date-parts":[[2023,10,18]]}}}