{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T14:08:34Z","timestamp":1776348514068,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AHRI core budget"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tuberculosis Research and Treatment"],"published-print":{"date-parts":[[2023,3,29]]},"abstract":"<jats:p>Background. Drug-resistant tuberculosis (TB) epidemic in high-TB-incidence countries, particularly Ethiopia, remains a significant challenge. As a result, we investigated the drug resistance, common gene mutation, and molecular characterization of mycobacterial isolates from patients with suspected tuberculous lymphadenitis (TBLN). Methodology. A cross-sectional study of 218 FNA samples from TBLN patients inoculated on Lowenstein-Jensen media was carried out. The culture isolates were identified as MTB by polymerase chain reaction (PCR) and the difference-9 (RD9) test region. In addition, the GenoType MTBDRplus assay tested the first and second-line MTB drugs, and the spoligotyping strain-dependent polymorphism test was determined. Results. Among the 50 culture-positive isolates, 14% (7\/50) had drug resistance caused by a gene mutation. Out of these, 4 (8%) isolates were mono-resistant to isoniazid drug, which is caused by a gene mutation in katG in the region of interrogated at codon 315 in the amino acid sequence of S315T1, and 3 (6%) isolates were resistant to both rifampicin and isoniazid drugs. The mutation was observed for katG (at codon 315 with a change in the sequence of amino acid S315T) and rpoB (at codon 530\u2013533 with a change in the sequence of amino acid S531L (S450L)) genes. The most prevalent spoligotypes were orphan and SIT53 strains. Conclusion. The predominance of INH mono-resistance poses a critical risk for the potential development of MDR-TB, as INH mono-resistance is a typical pathway to the occurrence of MDR-TB. The orphan and SIT53 (T) strains were the most common in the study area, and a drug-resistant strain caused by a common gene mutation could indicate the transmission of clonal-resistant strains in the community.<\/jats:p>","DOI":"10.1155\/2023\/3291538","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T21:50:45Z","timestamp":1680126645000},"page":"1-10","source":"Crossref","is-referenced-by-count":4,"title":["Drug Resistance in Tuberculous Lymphadenitis: Molecular Characterization"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1308-7566","authenticated-orcid":true,"given":"Gebeyehu","family":"Assefa","sequence":"first","affiliation":[{"name":"Armauer Hansen Research Institute, AHRI, Addis Ababa, Ethiopia"},{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, 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