{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T13:29:46Z","timestamp":1783344586300,"version":"3.54.6"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:00:00Z","timestamp":1675123200000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973801"],"award-info":[{"award-number":["81973801"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020"],"award-info":[{"award-number":["2020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. <jats:italic>QiHuangYiShen<\/jats:italic> granules (QHYS), a traditional Chinese herbal medicine formula, have been used in clinical practice for treating diabetic kidney disease for several years by our team. The efficacy of reducing proteinuria and delaying the decline of renal function of QHYS has been proved by our previous studies. However, the exact mechanism by which QHYS exerts its renoprotection remains largely unknown. Emerging evidence suggests that lncRNA MALAT1 is abnormally expressed in diabetic nephropathy (DN) and can attenuate renal fibrosis by modulating podocyte epithelial\u2010mesenchymal transition (EMT). <jats:italic>Objective<\/jats:italic>. In the present study, we aimed to explore whether QHYS could modulate lncRNA MALAT1 expression and attenuate the podocyte EMT as well as the potential mechanism related to the Wnt\/<jats:italic>\u03b2<\/jats:italic>\u2010catenin signal pathway. <jats:italic>Methods<\/jats:italic>. SD rats were fed with the high\u2010fat\u2010high\u2010sucrose diet for 8\u2009weeks and thereafter administered with 30\u2009mg\/kg streptozotocin intraperitoneally to replicate the DN model. Quality control of QHYS was performed using high\u2010performance liquid chromatography. QHYS were orally administered at 1.25, 2.5, and 5\u2009g\/kg doses, respectively, to the DN model rats for 12\u2009weeks. Body weight, glycated haemoglobin, blood urea nitrogen, serum creatinine, 24\u2010h proteinuria, and kidney index were measured. The morphologic pathology of the kidney was evaluated by Hematoxylin\u2010eosin and Masson\u2019s trichrome staining. The expression level of lncRNA MALAT1 was determined by quantitative real\u2010time polymerase chain reaction. In addition, the expression levels of podocyte EMT protein markers and Wnt\/<jats:italic>\u03b2<\/jats:italic>\u2010catenin pathway proteins in renal tissues were evaluated by Western blotting and immunohistochemistry. <jats:italic>Results<\/jats:italic>. The results showed that QHYS significantly reduced 24\u2010h proteinuria, blood urea nitrogen, kidney index, and ameliorated glomerular hypertrophy and collagen fiber deposition in the kidney of DN rats. Importantly, QHYS significantly downregulated the expression level of lncRNA MALAT1, upregulated the expression of nephrin, the podocyte marker protein, downregulated the expression of desmin and FSP\u20101, and mesenchymal cell markers. Furthermore, QHYS significantly downregulated the expression levels of Wnt1, <jats:italic>\u03b2<\/jats:italic>\u2010catenin, and active <jats:italic>\u03b2<\/jats:italic>\u2010catenin. <jats:italic>Conclusion<\/jats:italic>. Conclusively, our study revealed that QHYS significantly reduced proteinuria, alleviated renal fibrosis, and attenuated the podocyte EMT in DN rats, which may be associated with the downregulation of lncRNA MALAT1 expression and inhibition of the Wnt\/<jats:italic>\u03b2<\/jats:italic>\u2010catenin pathway.<\/jats:p>","DOI":"10.1155\/2023\/3357281","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T22:20:11Z","timestamp":1675203611000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>QiHuangYiShen<\/i> Granules Modulate the Expression of LncRNA MALAT1 and Attenuate Epithelial\u2010Mesenchymal Transition in Kidney of Diabetic Nephropathy Rats"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0864-4260","authenticated-orcid":false,"given":"Li-sha","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2875-992X","authenticated-orcid":false,"given":"Hua","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9177-9483","authenticated-orcid":false,"given":"Qiu-yue","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-0063","authenticated-orcid":false,"given":"Rong-lu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3918-951X","authenticated-orcid":false,"given":"Shi-wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9098-889X","authenticated-orcid":false,"given":"Shi-yi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-feng","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3135-2071","authenticated-orcid":false,"given":"Xiang-fei","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong-xia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-2979","authenticated-orcid":false,"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,31]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.2215\/cjn.11111016"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-7-415"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.2215\/cjn.11491116"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202009045"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.01.011"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4729019"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8400106"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118794"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13189"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/987064"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000410"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/2615286"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells4040631"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/0886022x.2017.1313164"},{"key":"e_1_2_10_15_2","unstructured":"YanP. 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