{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T14:06:00Z","timestamp":1768917960257,"version":"3.49.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012155","name":"National Institute for Medical Research Development","doi-asserted-by":"publisher","award":["982935"],"award-info":[{"award-number":["982935"]}],"id":[{"id":"10.13039\/501100012155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014588","name":"Sanofi Pasteur","doi-asserted-by":"publisher","award":["MEN30EXT"],"award-info":[{"award-number":["MEN30EXT"]}],"id":[{"id":"10.13039\/100014588","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>The death because of meningitis remains high in some parts of the world. It is important to know the specific cause of meningitis because the treatment differs depending on the cause. This study aimed to trace the false\u2010negative results of multiplex RT\u2010PCR to detect <jats:italic>Streptococcus pneumoniae<\/jats:italic>, <jats:italic>Haemophilus influenzae<\/jats:italic>, and <jats:italic>Neisseria meningitidis<\/jats:italic> serogroup by two different molecular methods. In this study, the CSF of the suspicious pediatric for acute bacterial meningitis among children aged 1\u2009month to 14\u2009years who are admitted to the hospitals in four cities of a certain region of Iran was collected. <jats:italic>S. pneumoniae<\/jats:italic>, <jats:italic>H. influenzae<\/jats:italic>, and <jats:italic>N. meningitidis<\/jats:italic> in CSF samples were detected by single\u2010tube multiplex RT\u2010PCR and specific RT\u2010PCR with a probe on the same specimens. In this cross\u2010sectional study, 506 CSF samples were collected during one year. The multiplex RT\u2010PCR can detect 3.3% and 2.2% of <jats:italic>S. pneumoniae<\/jats:italic> and <jats:italic>H. influenzae<\/jats:italic>, respectively. <jats:italic>N. meningitidis<\/jats:italic> was not detected. The CSF analysis was abnormal in 53% of 506 patients. On the other hand, 11.5%, 4.8%, and 4.1% of <jats:italic>S. pneumoniae<\/jats:italic>, <jats:italic>H. influenzae<\/jats:italic>, and <jats:italic>N. meningitidis<\/jats:italic> were identified, respectively, by specific RT\u2010PCR assay, exactly on the same specimens. Various types of PCR can be used for pathogen identification. As we change the type of PCR in our study, we could approximately increase 15% our positive results and also consequently decrease our false\u2010negative responses.<\/jats:p>","DOI":"10.1155\/2023\/3502666","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T17:50:08Z","timestamp":1673891408000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tracing the Negative Results of Multiplex Real\u2010Time PCR Assay for Diagnosis of Bacterial Pediatrics Meningitis"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4225-0097","authenticated-orcid":false,"given":"Abdollah","family":"Karimi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1723-9424","authenticated-orcid":false,"given":"Sedigheh","family":"Rafiei 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Tehrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5902-0845","authenticated-orcid":false,"given":"Fatemeh","family":"Fallah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iman","family":"Faghihian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,16]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.7754\/clin.lab.2016.160912"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1478-7954-11-17"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198772"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.2217\/bmm.14.67"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.9650"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019361"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.06087-11"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.39.4.1553-1558.2001"},{"key":"e_1_2_11_9_2","unstructured":"CDC 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