{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:12:16Z","timestamp":1772964736218,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":143,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018227","name":"National Research Foundation of Ukraine","doi-asserted-by":"publisher","award":["2020.01\/0204"],"award-info":[{"award-number":["2020.01\/0204"]}],"id":[{"id":"10.13039\/100018227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. In the present work, we investigated the effect of exogenous glutathione in old rats on the expression of ATP\u2010sensitive potassium (K<jats:sub>ATP<\/jats:sub>) channels, the mitochondrial permeability transition pore (mPTP) opening in the heart, and the vasorelaxation responses of isolated aortic rings to activation of K<jats:sub>ATP<\/jats:sub> channels. <jats:italic>Methods<\/jats:italic>. Experiments were performed on adult (6 months) and old (24 months) male Wistar rats, which were divided into three groups: adult, old, and glutathione\u2010treated old rats. Glutathione was injected intraperitoneally at a dose of 52\u2009mg\/kg 1 hour before the studies. The mRNA expression of K<jats:sub>ATP<\/jats:sub> channels was determined using reverse transcription and real\u2010time polymerase chain reaction analysis. The effect of glutathione administration on mPTP opening, relaxation responses of isolated aortic rings, and oxidative stress markers was studied. <jats:italic>Results<\/jats:italic>. It was shown that the expression levels of Kir6.1, Kir6.2, and SUR1 subunits of K<jats:sub>ATP<\/jats:sub> channels and levels of reduced glutathione were significantly increased in glutathione\u2010treated old rats (by 8.3, 2.8, 13.1, and 1.5\u2010fold, respectively), whereas the levels of oxidative stress markers (hydrogen peroxide, diene conjugates, malondialdehyde, and rate of superoxide generation) in heart mitochondria and mPTP opening were significantly reduced. Relaxation of aortic rings was significantly increased in response to the actions of K<jats:sub>ATP<\/jats:sub> channel openers flocalin and pinacidil in glutathione\u2010treated animals, which was prevented by glibenclamide. <jats:italic>Conclusions<\/jats:italic>. Thus, the administration of exogenous glutathione to old rats resulted in a significant increase in the expression levels of the Kir6.1, Kir6.2, and SUR1 subunits of K<jats:sub>ATP<\/jats:sub> channels and a decrease in oxidative stress. This was accompanied by inhibition of mPTP opening and enhancement of vasorelaxation responses to activation of K<jats:sub>ATP<\/jats:sub> channels.<\/jats:p>","DOI":"10.1155\/2023\/3562847","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T22:35:09Z","timestamp":1684967709000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Glutathione Upregulates the Expression of K<sub>ATP<\/sub> Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2189-7932","authenticated-orcid":false,"given":"Ruslan","family":"Strutynskyi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natali\u0456a","family":"Strutynska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3943-4768","authenticated-orcid":false,"given":"Lidiia","family":"Mys","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulia","family":"Goshovska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuliia","family":"Korkach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raisa","family":"Fedichkina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iryna","family":"Okhai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladyslav","family":"Strutynskyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadym","family":"Sagach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389200216666151015114515"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.3.489"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9100909"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04711"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0771-y"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13829"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11050801"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2020.05.014"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13683"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-021-04237-8"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-014-1227-1"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13513"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2009.134"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz66.06.066"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.01072.x"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz56.04.059"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.378"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz57.01.055"},{"key":"e_1_2_10_19_2","first-page":"15","article-title":"Cardioprotective effects of flokalin: relative role of activation of sarcolemmal and mitochondrial adenosine triphosphate-dependent potassium channels","volume":"54","author":"Strutyns\u2032ky\u012d R. B.","year":"2008","journal-title":"Fiziolohichnyi Zhurnal"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz58.01.013"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz59.04.016"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00552.2001"},{"key":"e_1_2_10_23_2","first-page":"12","article-title":"Cardioprotective effects of activation of ATP-sensitive potassium channels in experiments in vivo: influence on blood biochemical parameters following ischemia-reperfusion of the myocardium","volume":"55","author":"Strutyns\u2019kyi R. B.","year":"2009","journal-title":"Fiziolohichnyi Zhurnal"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz59.03.003"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.07.007"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303051200"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5483com"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-1602fje"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000394"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2000.5979"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.15407\/fz59.01.003"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1093388"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(01)01428-0"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201806_15181"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25003"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2023\/3562847.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2023\/3562847.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2023\/3562847","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T23:27:48Z","timestamp":1722900468000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2023\/3562847"}},"subtitle":[],"editor":[{"given":"Hu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1155\/2023\/3562847"],"URL":"https:\/\/doi.org\/10.1155\/2023\/3562847","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"2022-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3562847"}}