{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:36:31Z","timestamp":1790732191047,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T00:00:00Z","timestamp":1677456000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011073","name":"VetAgro Sup","doi-asserted-by":"publisher","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}],"id":[{"id":"10.13039\/501100011073","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012983","name":"Universit\u00e9 Saint-Esprit de Kaslik","doi-asserted-by":"publisher","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}],"id":[{"id":"10.13039\/100012983","id-type":"DOI","asserted-by":"publisher"}]},{"name":"French Embassy","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}]},{"name":"Directorate General for Food","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}]},{"DOI":"10.13039\/501100002708","name":"Agence Universitaire de la Francophonie","doi-asserted-by":"publisher","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}],"id":[{"id":"10.13039\/501100002708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007175","name":"CNRS-L","doi-asserted-by":"crossref","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}],"id":[{"id":"10.13039\/501100007175","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PHC-C\u00e8dre","award":["47858PK"],"award-info":[{"award-number":["47858PK"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2023,2,27]]},"abstract":"<jats:p>Leptospirosis is a worldwide bacterial zoonosis for which the risk increases in warm and wet climates. Despite the suitability of the local climate for the bacteria\u2019s persistence, Lebanon lacks a formal system of prophylaxis for the prevention of Leptospira infection in both humans and animals, and the disease\u2019s epidemiology is unknown so far. As a preliminary step, we focused on Leptospira infection in cattle, which is of public health and economic concern. We conducted a descriptive study in cattle from the governorate of Mount Lebanon (ML) and in imported cattle. A total of 187 blood and 135 serum samples were provided. Among the 187 blood samples, 135 were from randomly selected animals from 14 herds, while the remaining 52 were from imported livestock. Serum specimens (n\u2009=\u2009135) were obtained exclusively from cattle in the ML governorate. DNA was extracted from all blood samples and subjected to real-time PCR targeting 16S rRNA. All Leptospira-positive DNA samples were then amplified using conventional PCR (cPCR), and Leptospira species were identified via Sanger sequencing. A microscopic agglutination test (MAT) was performed on the 135 serum samples from local cattle. The real-time PCR revealed Leptospira infection in 7 of 135 local animals and 1 of 52 imported animals. DNA from six of the seven local animals and the one imported animal were amplified by cPCR and successfully sequenced, identifying the pathogen as the species L. kirschneri. Seven animals located in five out of the 14 tested herds had MAT titers \u22651\u2009:\u2009100. Serogroup Grippotyphosa was predominant. This is the first study to provide epidemiological data on bovine Leptospira infection in Lebanon. Pathogenic Leptospira species and serogroups were identified in both local and imported cattle. These findings highlight the cattle associated risk of Leptospira infection in Lebanon, in the contexts of farming and trade. Summary. Leptospirosis is a severe zoonotic disease that can have critical consequences for people and animals. Within the country of Lebanon, this disease has been reported, but its epidemiology is unknown so far. The present study thus provides the first description of the existence of Leptospira in cattle in Lebanon (local and imported). It also highlights the existence of different pathogenic serogroups of Leptospira in local cattle. Our results should raise public health awareness of the threat posed by this underdiagnosed disease and serve as a starting point for control efforts in Lebanon.<\/jats:p>","DOI":"10.1155\/2023\/3784416","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T03:20:05Z","timestamp":1677554405000},"page":"1-10","source":"Crossref","is-referenced-by-count":4,"title":["Molecular and Serological Identification of Pathogenic Leptospira in Local and Imported Cattle from Lebanon"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1674-8817","authenticated-orcid":true,"given":"Elena","family":"Harran","sequence":"first","affiliation":[{"name":"VetAgro Sup, Universit\u00e9 Claude Bernard Lyon 1, USC 1233, Marcy L\u2019Etoile, Lyon, France"},{"name":"Faculty of Arts and Sciences, Holy Spirit University of Kaslik (USEK), P.O. Box 446, Jounieh, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Abi Rizk","sequence":"additional","affiliation":[{"name":"Faculty of Arts and Sciences, Holy Spirit University of Kaslik (USEK), P.O. Box 446, Jounieh, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Angelloz-Pessey","sequence":"additional","affiliation":[{"name":"VetAgro Sup, Universit\u00e9 Claude Bernard Lyon 1, Laboratoire des Leptospires et d\u2019Analyses V\u00e9t\u00e9rinaires, Marcy L\u2019Etoile, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Groud","sequence":"additional","affiliation":[{"name":"VetAgro Sup, Universit\u00e9 Claude Bernard Lyon 1, USC 1233, Marcy L\u2019Etoile, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Lattard","sequence":"additional","affiliation":[{"name":"VetAgro Sup, Universit\u00e9 Claude Bernard Lyon 1, USC 1233, Marcy L\u2019Etoile, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christo","family":"Hilan","sequence":"additional","affiliation":[{"name":"Faculty of Arts and Sciences, Holy Spirit University of Kaslik (USEK), P.O. 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