{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:00:02Z","timestamp":1740139202550,"version":"3.37.3"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"],"award-info":[{"award-number":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nanning Scientific Research and Technology Development Project","award":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"],"award-info":[{"award-number":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"]}]},{"DOI":"10.13039\/501100004924","name":"Guangxi Traditional Chinese Medical University","doi-asserted-by":"publisher","award":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"],"award-info":[{"award-number":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"]}],"id":[{"id":"10.13039\/501100004924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"],"award-info":[{"award-number":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangxi Zhuang Autonomous Region Health Commission Scientific Research","award":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"],"award-info":[{"award-number":["2016GXNSFAA380234","20173017-8","2018BS036","2020DXS58","82060286","Z20210107","Z20190633"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Clinical Practice"],"published-print":{"date-parts":[[2023,8,23]]},"abstract":"<jats:p>Background. Leptin (LEP) is believed to play a crucial role in male reproduction, while the molecular mechanisms through which LEP affects the male reproductive system are unclear. LEP acts by binding to a leptin receptor (LEPR) which mediates its physiological action, but there are only limited studies on the function of LEPR in human sperm. Purpose. This study aimed to determine the Gln223Arg polymorphisms of the LEPR gene in human spermatozoa and evaluate their possible relationship with semen variables. Methods. The study was performed on Chinese men: 115 healthy subjects and 108 patients with primary and 98 with secondary infertility. Semen samples were obtained from all patients, and semen variables were analyzed. The genotypic and allelic frequencies of Gln223Arg polymorphism in spermatozoa were determined by PCR and restriction fragment length polymorphism (RFLP) analyses. Statistical analyses were performed using the chi-square test, the Kruskal\u2013Wallis test, and the Mann\u2013Whitney test. Results. There were no significant differences in genotypic or allelic frequency distributions of Gln223Arg polymorphism among men with primary infertility, secondary infertility, and controls. Similarly, semen volume and sperm concentration did not differ with the different genotypes in all groups of men. The percentages of motile sperm for AA\u2009+\u2009AG genotypes in men with primary infertility (31.98%) were significantly lower than those in secondary infertility, and control men with GG genotypes were 34.41% and 59.36%, respectively. At the same time, the percentages of normal morphology sperm for AA\u2009+\u2009AG genotypes in men with primary infertility (2.93%) were significantly lower than those in secondary infertility and control men with GG genotypes 3.71% and 6.54%, respectively. Conclusion. This study reveals a possible association between the Gln223Arg polymorphism of the LEPR gene in spermatozoa affecting spermatozoal membrane integrity and having a direct role in sperm motility.<\/jats:p>","DOI":"10.1155\/2023\/4009061","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T21:05:06Z","timestamp":1692824706000},"page":"1-8","source":"Crossref","is-referenced-by-count":2,"title":["Leptin Receptor Gln223Arg Polymorphism of Human Spermatozoa Associated with Male Infertility in a Chinese Population"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6857-9010","authenticated-orcid":true,"given":"Yi","family":"Mo","sequence":"first","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4444-9657","authenticated-orcid":true,"given":"Fangfang","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7547-4767","authenticated-orcid":true,"given":"Arshad","family":"Mehmood","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suleman","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, School of Basic Medical Sciences, Health Science Center, Shenzhen University, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Genetics, Hebei Medical University, Hebei Key Lab of Laboratory Animal, Shijiazhuang 050017, Hebei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong","family":"Lin","sequence":"additional","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hesheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafen","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangli","family":"Niu","sequence":"additional","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinggan","family":"Mo","sequence":"additional","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"4","key":"1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/humupd\/dmp012","article-title":"Approaches to improve the diagnosis and management of infertility","volume":"15","author":"P. Devroey","year":"2009","journal-title":"Human Reproduction Update"},{"issue":"4","key":"2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/S1472-6483(10)60457-9","article-title":"Gene polymorphisms\/mutations relevant to abnormal spermatogenesis","volume":"16","author":"F. Nuti","year":"2008","journal-title":"Reproductive BioMedicine Online"},{"issue":"1","key":"3","doi-asserted-by":"crossref","DOI":"10.1186\/1477-7827-12-15","article-title":"Hyperleptinemia directly affects testicular maturation at different sexual stages in mice, and suppressor of cytokine signaling 3 is involved in this process","volume":"12","author":"M. Yuan","year":"2014","journal-title":"Reproductive Biology and Endocrinology"},{"issue":"8","key":"4","doi-asserted-by":"crossref","first-page":"2781","DOI":"10.1210\/en.2014-1119","article-title":"Leptin monotherapy rescues spermatogenesis in male Akita type 1 diabetic mice","volume":"155","author":"E. L. Schoeller","year":"2014","journal-title":"Endocrinology"},{"issue":"7","key":"5","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2174\/1381612822666151209151550","article-title":"Male fertility and obesity: are ghrelin, leptin and glucagon-like peptide-1 pharmacologically relevant?","volume":"22","author":"M. G. Alves","year":"2016","journal-title":"Current Pharmaceutical Design"},{"issue":"1","key":"6","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/andr.12295","article-title":"Genetic variation in leptin and leptin receptor genes as a risk factor for idiopathic male infertility","volume":"5","author":"A. Hod\u017ei\u0107","year":"2017","journal-title":"Andrology"},{"issue":"1","key":"7","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.metabol.2014.10.015","article-title":"Leptin resistance and diet-induced obesity: central and peripheral actions of leptin","volume":"64","author":"N. S\u00e1inz","year":"2015","journal-title":"Metabolism"},{"issue":"6","key":"8","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1007\/s00404-012-2675-x","article-title":"Relationship of leptin administration with production of reactive oxygen species, sperm DNA fragmentation, sperm parameters and hormone profile in the adult rat","volume":"287","author":"S. Abbasihormozi","year":"2013","journal-title":"Archives of Gynecology and Obstetrics"},{"issue":"6","key":"9","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1365-2605.2003.00434.x","article-title":"Leptin and leptin receptor in human seminal plasma and in human spermatozoa","volume":"26","author":"T. Jope","year":"2003","journal-title":"International Journal of Andrology"},{"issue":"1","key":"10","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1439-0272.2006.00754.x","article-title":"Expression of leptin and leptin receptor in the testis of fertile and infertile patients","volume":"39","author":"T. Ishikawa","year":"2007","journal-title":"Andrologia"},{"issue":"5","key":"11","first-page":"7065","article-title":"Role of leptin and the leptin receptor in the pathogenesis of varicocele-induced testicular dysfunction","volume":"17","author":"J. Zhang","year":"2018","journal-title":"Molecular Medicine Reports"},{"issue":"6","key":"12","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/j.1365-2605.2008.00931.x","article-title":"Effect of leptin on motility, capacitation and acrosome reaction of human spermatozoa","volume":"32","author":"H. W. Li","year":"2009","journal-title":"International Journal of Andrology"},{"issue":"10","key":"13","first-page":"3550","article-title":"Sperm motility inversely correlates with seminal leptin levels in idiopathic asthenozoospermia","volume":"7","author":"J. Guo","year":"2014","journal-title":"International Journal of Clinical and Experimental Medicine"},{"issue":"3","key":"14","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1210\/endo.138.3.5024","article-title":"Leptin treatment rescues the sterility of genetically obese ob\/ob males","volume":"138","author":"K. Mounzih","year":"1997","journal-title":"Endocrinology"},{"issue":"6674","key":"15","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1038\/32911","article-title":"A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction","volume":"392","author":"K. Cl\u00e9ment","year":"1998","journal-title":"Nature"},{"issue":"2","key":"16","doi-asserted-by":"crossref","first-page":"302","DOI":"10.2164\/jandrol.05133","article-title":"Influence of a leptin deficiency on testicular morphology, germ cell apoptosis, and expression levels of apoptosis-related genes in the mouse","volume":"27","author":"G. K. Bhat","year":"2006","journal-title":"Journal of Andrology"},{"issue":"5","key":"17","doi-asserted-by":"crossref","first-page":"R159","DOI":"10.1530\/REP-15-0261","article-title":"Genetics of male infertility: from research to clinic","volume":"150","author":"C. Krausz","year":"2015","journal-title":"Reproduction"},{"issue":"1","key":"18","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1080\/19396368.2016.1258741","article-title":"Leptin and leptin-receptor polymorphisms in fertile and infertile men","volume":"63","author":"S. Khosropour","year":"2017","journal-title":"Systems Biology in Reproductive Medicine"},{"issue":"10","key":"19","first-page":"867","article-title":"[WHO laboratory manual for the examination and processing of human semen: its applicability to andrology laboratories in China]","volume":"16","author":"J. C. Lu","year":"2010","journal-title":"Zhonghua Nan ke Xue"},{"issue":"2","key":"20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S1665-2681(19)32025-3","article-title":"Obesity-related leptin receptor polymorphisms and gallstones disease","volume":"5","author":"N. M\u00e9ndez-S\u00e1nchez","year":"2006","journal-title":"Annals of Hepatology"},{"issue":"3","key":"21","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s10549-009-0503-1","article-title":"Common genetic variations in the LEP and LEPR genes, obesity and breast cancer incidence and survival","volume":"120","author":"R. J. Cleveland","year":"2010","journal-title":"Breast Cancer Research and Treatment"},{"issue":"2","key":"22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/aje\/kwi174","article-title":"Genetics of leptin and obesity: a HuGE review","volume":"162","author":"V. Paracchini","year":"2005","journal-title":"American Journal of Epidemiology"},{"issue":"1","key":"23","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1186\/1471-2407-8-338","article-title":"Leptin receptor Gln223Arg polymorphism and breast cancer risk in Nigerian women: a case control study","volume":"8","author":"M. N. Okobia","year":"2008","journal-title":"BMC Cancer"},{"issue":"3","key":"24","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s004390100468","article-title":"A single nucleotide polymorphism (SNP) in the leptin receptor is associated with BMI, fat mass and leptin levels in postmenopausal Caucasian women","volume":"108","author":"N. D. Quinton","year":"2001","journal-title":"Human Genetics"},{"issue":"4","key":"25","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1097\/JCMA.0000000000000507","article-title":"Association of insulin resistance and leptin receptor gene polymorphism in type 2 diabetes mellitus","volume":"84","author":"U. Adiga","year":"1 2021","journal-title":"Journal of the Chinese Medical Association"},{"key":"26","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-3-030-37972-8","volume-title":"Genetics of Male Infertility: A Case-Based Guide for Clinicians","author":"M. Arafa","year":"2020","edition":"1st"},{"issue":"1","key":"27","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1006\/bbrc.1996.1133","article-title":"Molecular cloning of rat leptin receptor isoform complementary DNAs--identification of a missense mutation in Zucker fatty (fa\/fa) rats","volume":"225","author":"K. Takaya","year":"1996","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"2","key":"28","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1006\/bbrc.1997.7205","article-title":"Detection and quantification of the leptin receptor splice variants Ob-Ra, b, and, e in different mouse tissues","volume":"238","author":"B. L\u00f6llmann","year":"1997","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"10","key":"29","doi-asserted-by":"crossref","first-page":"6093","DOI":"10.1074\/jbc.272.10.6093","article-title":"The leptin receptor","volume":"272","author":"L. A. Tartaglia","year":"1997","journal-title":"Journal of Biological Chemistry"},{"issue":"3","key":"30","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1093\/humupd\/dmv057","article-title":"Leptin and its potential interest in assisted reproduction cycles","volume":"22","author":"A. Catteau","year":"2016","journal-title":"Human Reproduction Update"}],"container-title":["International Journal of Clinical Practice"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijclp\/2023\/4009061.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijclp\/2023\/4009061.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijclp\/2023\/4009061.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T21:05:26Z","timestamp":1692824726000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijclp\/2023\/4009061\/"}},"subtitle":[],"editor":[{"given":"Samar","family":"Tharwat","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,8,23]]},"references-count":30,"alternative-id":["4009061","4009061"],"URL":"https:\/\/doi.org\/10.1155\/2023\/4009061","relation":{},"ISSN":["1742-1241","1368-5031"],"issn-type":[{"type":"electronic","value":"1742-1241"},{"type":"print","value":"1368-5031"}],"subject":[],"published":{"date-parts":[[2023,8,23]]}}}