{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T20:09:09Z","timestamp":1774987749129,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008853","name":"Jining Medical University","doi-asserted-by":"publisher","award":["2020-BS-014"],"award-info":[{"award-number":["2020-BS-014"]}],"id":[{"id":"10.13039\/501100008853","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008853","name":"Jining Medical University","doi-asserted-by":"publisher","award":["M-2022246"],"award-info":[{"award-number":["M-2022246"]}],"id":[{"id":"10.13039\/501100008853","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shandong Province Chinese Medicine Science and Technology Project","award":["2020-BS-014"],"award-info":[{"award-number":["2020-BS-014"]}]},{"name":"Shandong Province Chinese Medicine Science and Technology Project","award":["M-2022246"],"award-info":[{"award-number":["M-2022246"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2023,8,16]]},"abstract":"<jats:p>Background\/Aims. Accumulating clinical evidence suggests that Colquhounia root tablet (CRT) has the potential to alleviate diabetic nephropathy (DN); however, the exact mechanism of action remains unclear. Here, we report the effects of CRT administration on apoptosis and autophagy and attempt to elucidate the underlying mechanisms in vivo and in vitro. Methods. Rat models of DN were established using streptozotocin (STZ). The primary metabolic parameters were assessed. The pathological changes of the rat kidney were investigated, and RNA sequencing was performed for each group. Renal tissue apoptosis was detected using the TUNEL assay. In rats and high glucose- (Hg-) induced HK-2 cells, RT-qPCR and western blot were used to analyze the expression of related genes and proteins. Hg medium was used to establish the diabetic kidney environment. The CCK-8 assay and flow cytometry were used to assess cell viability and apoptosis, respectively. Transmission electron microscopy was used to evaluate autophagy in vitro. Results. CRT treatment significantly reduced albuminuria and renal tissue damage in DN rats. Furthermore, CRT administration inhibited apoptosis and promoted autophagy in DN rat kidney tissues. CRT downregulated CD36 expression and activated the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway in DN rat kidney tissues. CRT intervention inhibited Hg-induced apoptosis and reversed autophagy in HK-2 cells. Moreover, overexpression of CD36 suppressed the beneficial effects of CRT. Conclusions. Our study is the first to report that CRT inhibited apoptosis and promoted autophagy in vivo and in vitro, which was achieved by reducing CD36 expression and activating the AMPK pathway. Therefore, CRT may be an effective drug to treat DN.<\/jats:p>","DOI":"10.1155\/2023\/4617653","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T18:35:34Z","timestamp":1692210934000},"page":"1-11","source":"Crossref","is-referenced-by-count":7,"title":["Colquhounia Root Tablet Promotes Autophagy and Inhibits Apoptosis in Diabetic Nephropathy by Suppressing CD36 Expression In Vivo and In Vitro"],"prefix":"10.1155","volume":"2023","author":[{"given":"Han","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine, Jining Medical University, Jining, Shandong 272013, China"},{"name":"Department of General Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baiju","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Jining Medical University, Jining, Shandong 272013, China"},{"name":"Department of General Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changbao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Jining Medical University, Jining, Shandong 272013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Jining Medical University, Jining, Shandong 272013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jizhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Wenshang County People\u2019s Hospital, Jining, Shandong 272501, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5774-978X","authenticated-orcid":true,"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.4414\/smw.2017.14458"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-018-0001-y"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27427"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/2615286"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-58781-2"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/1497449"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-019-0234-4"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X22500744"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1024634"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115358"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.1674-6376.2021.09.032"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbl2703094"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-021-00563-7"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13534"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S237451"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1080\/0886022X.2020.1869043"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1004-2407.2022.05.010"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-020-02545-4"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.4161\/auto.6260"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2017.126"},{"issue":"3","key":"21","first-page":"248","article-title":"Studies on the effect of pretreatment of Colquhounia Root on acute lung injury in rats caused by oleic acid","volume":"36","author":"X. G. Ma","year":"2014","journal-title":"Journal of Ningxia Medical University"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2697672"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.13286\/j.cnki.chinhosppharmacyj.2014.20.17"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1004-0188.2016.09.001"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3390\/biom12071010"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.111771"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-020-0309-2"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1177-1"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-021-00721-5"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201801711R"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2022.08.012"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014050457"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2012.11.004"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127507"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.P040501"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M090969"}],"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2023\/4617653.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2023\/4617653.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2023\/4617653.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T18:37:47Z","timestamp":1692211067000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jdr\/2023\/4617653\/"}},"subtitle":[],"editor":[{"given":"Ilaria","family":"Campesi","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,8,16]]},"references-count":36,"alternative-id":["4617653","4617653"],"URL":"https:\/\/doi.org\/10.1155\/2023\/4617653","relation":{},"ISSN":["2314-6753","2314-6745"],"issn-type":[{"value":"2314-6753","type":"electronic"},{"value":"2314-6745","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8,16]]}}}