{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:15:53Z","timestamp":1740147353637,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001","2021\/09272-0","2019\/00204-1"],"award-info":[{"award-number":["001","2021\/09272-0","2019\/00204-1"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["001","2021\/09272-0","2019\/00204-1"],"award-info":[{"award-number":["001","2021\/09272-0","2019\/00204-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scanning"],"published-print":{"date-parts":[[2023,4,17]]},"abstract":"<jats:p>The aim of this study was to evaluate the sensitivity, specificity, and predictive values of the fluorescence microscopy method in the detection of apical dental reabsorption after induction of apical periodontitis in animal models. Forty-first molars of mice, aged 6 to 8 weeks, had their root canals exposed to the oral environment or were maintained healthy as controls (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>n<\/a:mi>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>20<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). After 14 and 42 days, mice were euthanized and tissues were collected for histological evaluation by means of bright field and fluorescence microscopy. The accuracy of fluorescence microscopy in identifying apical external dental resorption was investigated using a diagnostic validation test based on the sensitivity (S) and specificity (E) properties. Bright-field microscopy revealed a higher number of specimens with scores of 1 to 3 - absence of apical dental resorption (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>n<\/c:mi>\n                        <c:mo>=<\/c:mo>\n                        <c:mn>29<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>; 52%), while fluorescence microscopy revealed a higher number of specimens with scores of 4 to 6 - presence of apical dental resorption (<jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>n<\/e:mi>\n                        <e:mo>=<\/e:mo>\n                        <e:mn>37<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>; 66%). Out of 56 specimens, 26 were TP, 11 were FP, and 19 were TN. No FN result was observed. Fluorescence microscopy presented a sensitivity value of 1, similar to the bright-field method, while specificity was lower (0.633). The accuracy of the fluorescent method to detect apical dental resorption was 0.804. Fluorescence microscopy revealed a higher number of false positive apical dental resorption than bright-field microscopy. The detection of apical dental resorption was not impacted by the sensitivity of the method but by its specificity.<\/jats:p>","DOI":"10.1155\/2023\/4619503","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T17:51:31Z","timestamp":1681753891000},"page":"1-4","source":"Crossref","is-referenced-by-count":0,"title":["Detection of Apical Dental Resorption Caused by Endodontic Infection in Mice Using Fluorescence and Bright-Field Microscopy"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7659-7673","authenticated-orcid":true,"given":"Marcio Santos","family":"de Carvalho","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4464-7506","authenticated-orcid":true,"given":"Luciano Aparecido","family":"de Almeida-Junior","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5858-4318","authenticated-orcid":true,"given":"Yuri Jivago Silva","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1044-0937","authenticated-orcid":true,"given":"Maya Fernanda Manfrin","family":"Arnez","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0230-1347","authenticated-orcid":true,"given":"Raquel Assed Bezerra","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4837-0583","authenticated-orcid":true,"given":"Fabr\u00edcio Kitazono","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-532X","authenticated-orcid":true,"given":"Francisco Wanderley Garcia","family":"Paula-Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","first-page":"48","article-title":"Glossary of Endodontic Terms","volume":"10","author":"American Association of Endodontists","year":"2020","journal-title":"Chicago"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.05.009"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.07.028"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107bor-2018.vol32.0069"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2006.05.027"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.10.001"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.10.036"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12650"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2000.00338.x"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-77572011005000020"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1177\/1066896913517939"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1259\/dmfr\/93927281"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2008.12.009"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.01.003"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2022.08.003"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-014-1340-0"},{"volume-title":"ROC analysis: web-based calculator for ROC curves","year":"2021","author":"J. 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