{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:43:33Z","timestamp":1770889413379,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"R&D Program of Zhejiang","award":["2022C03002"],"award-info":[{"award-number":["2022C03002"]}]},{"name":"R&D Program of Zhejiang","award":["2022KY075"],"award-info":[{"award-number":["2022KY075"]}]},{"name":"R&D Program of Zhejiang","award":["2023KY489"],"award-info":[{"award-number":["2023KY489"]}]},{"name":"Zhejiang Provincial Medical and Health Science and Technology","award":["2022C03002"],"award-info":[{"award-number":["2022C03002"]}]},{"name":"Zhejiang Provincial Medical and Health Science and Technology","award":["2022KY075"],"award-info":[{"award-number":["2022KY075"]}]},{"name":"Zhejiang Provincial Medical and Health Science and Technology","award":["2023KY489"],"award-info":[{"award-number":["2023KY489"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2023,9,27]]},"abstract":"<jats:p>Rapid and accurate methods for the diagnosis of tuberculous pleurisy (TP) are urgently needed. Activation markers of tuberculosis (TB)\u2013reactive T cells are considered promising for the diagnosis of active TB (ATB). Different activation indexes may play different roles in the progression of TB, but there are few reports on T cell activation indicators, except for HLA-DR. Hence, we evaluated the expression of early (CD25 and CD69) and late (CD134) activation markers on TB antigen-stimulated CD4+ T cells in populations with different TB infection status and investigated their diagnostic value for ATB, particularly, for TP. Moreover, we compared the differences in the diagnostic efficacy among the indexes from peripheral blood (PB) and pleural fluid (PF) for TP. The expression of each activation marker was significantly increased in TB-infected populations (patients with ATB and latent TB infection vs. healthy individuals; patients with TP vs. non-TP) and was significantly higher in the PF than in the PB of patients with TP. The diagnostic performance of the coexpressed activation markers was superior to that of single expression markers in the differential diagnosis of ATB and non-TB, with CD25+CD134+ showing the best diagnostic efficiency (AUC: 0.93, 95% CI, 0.87\u20130.99; sensitivity: 86.7%, 95% CI, 72.5%\u201394.5%; and specificity: 94.0%, 95% CI, 82.5%\u201398.4%). Except for TB-IGRA, the activation indexes were more accurate than conventional laboratory methods for ATB diagnosis. In addition, the expression of CD25+CD134+ in PB and PF was the best values for differential diagnosis of TP and NTP, with AUCs of 0.87 (95% CI, 0.77\u20130.96) and 0.95 (95% CI, 0.90\u20131.00), respectively. Our study provides information on the diagnostic value of different activation markers for TB and shows that the expression of CD25+CD134+ on CD4+ T cells in PF can serve as a potential marker for TP diagnosis.<\/jats:p>","DOI":"10.1155\/2023\/5309816","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T19:44:18Z","timestamp":1695843858000},"page":"1-12","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Value of CD25, CD69, and CD134 on Tuberculosis-Specific Antigen-Stimulated CD4+ T Cells for Tuberculous Pleurisy"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6021-900X","authenticated-orcid":true,"given":"Hanlu","family":"Shi","sequence":"first","affiliation":[{"name":"Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College), Hangzhou 310014, China"},{"name":"School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Yang","sequence":"additional","affiliation":[{"name":"The Quzhou Hospital of Wenzhou Medical University, Quzhou People\u2019s Hospital, Quzhou 324000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fujie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qian Xi Nan Hospital of Traditional Chinese Medicine, Qian Xi Nan Buyei and Miao Autonomous Prefecture, Guizhou 562499, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5284-7797","authenticated-orcid":true,"given":"Yu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College), Hangzhou 310014, China"},{"name":"Key Laboratory of Biomarkers and in vitro Diagnosis Translation of Zhejiang Province, Hangzhou, Zhejiang 310063, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9394-7791","authenticated-orcid":true,"given":"Yonglie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College), Hangzhou 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","author":"W. 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