{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:11:17Z","timestamp":1753888277400,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":178,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Chronic alcohol consumption damages bone formation and causes bone pathology, including osteonecrosis of the femoral head. The aim of this work was to evaluate the effects of the leaf aqueous extract of <jats:italic>Chromolaena odorata<\/jats:italic> (<jats:italic>C. odorata<\/jats:italic>) on the femoral head in ethanol\u2010induced osteonecrosis in rats. Animals received alcohol (40\u00b0) at 3\u2009g\/kg for 12\u2009weeks. A group of animals were sacrificed to attest to the instalment of osteonecrosis by using histopathological analysis. The remaining animals received alcohol concomitantly with the plant extract (150, 300, or 600\u2009mg\/kg) or diclofenac (1\u2009mg\/kg) for 28 additional days. At the end of the experimental period, biochemical parameters including total cholesterol, triglycerides, calcium, alkaline phosphatase (ALP), reduced glutathione (GSH), malondialdehyde (MDA), nitrite, superoxide dismutase (SOD), and catalase activities were measured. Histopathological and histomorphometry analyses of femurs were also assessed. The administration of alcohol, irrespective of the experimental period, induced a significant increase in total cholesterol (<jats:italic>p<\/jats:italic> &lt; 0.05) and triglyceride (<jats:italic>p<\/jats:italic> &lt; 0.01) and a decrease in ALP (<jats:italic>p<\/jats:italic> &lt; 0.05) and calcium (<jats:italic>p<\/jats:italic> &lt; 0.05\u2013<jats:italic>p<\/jats:italic> &lt; 0.001) levels. Intoxicated animals showed an alteration in oxidative stress parameters accompanied by a significant drop in bone cortical thickness and density with necrosis and marked bone resorption. The concomitant administration of the plant with ethanol reversed the alcohol\u2010induced bone defect, characterized by the improvement of the lipid profile (<jats:italic>p<\/jats:italic> &lt; 0.001), bone calcium concentration (<jats:italic>p<\/jats:italic> &lt; 0.05), bone ALP activity (<jats:italic>p<\/jats:italic> &lt; 0.001), oxidative stress parameters, improved cortical bone thickness (<jats:italic>p<\/jats:italic> &lt; 0.01), and bone density (<jats:italic>p<\/jats:italic> &lt; 0.05). These results are supported by the absence of bone resorption with an obvious effect at a dose of 300\u2009mg\/kg. The pharmacological effect of the extract on ethanol\u2010induced osteonecrosis of the femoral head is probably due to its osteogenic, hypolipidemic, and antioxidant properties, justifying its use in Cameroonian folk medicine for articulation and bone pain management.<\/jats:p>","DOI":"10.1155\/2023\/5436771","type":"journal-article","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T17:35:17Z","timestamp":1687973717000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Chromolaena odorata<\/i> (L.) R. M. King and H. Robinson Leaves Aqueous Extract Improves the Femoral Head in Ethanol\u2010Induced Osteonecrosis in Rats"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5382-9184","authenticated-orcid":false,"given":"Florence","family":"Tsofack Ngueguim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5685-9285","authenticated-orcid":false,"given":"Raceline","family":"Kamkumo Gounoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Hubert Donfack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8143-1817","authenticated-orcid":false,"given":"Sandra","family":"Manefen Simo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5435-9787","authenticated-orcid":false,"given":"Josiane","family":"Jouonzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0893-5260","authenticated-orcid":false,"given":"Rodrigue","family":"Ngapout Fifen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5315-6285","authenticated-orcid":false,"given":"Paul Desire","family":"Djomeni Dzeufiet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8565-1181","authenticated-orcid":false,"given":"Th\u00e9ophile","family":"Dimo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109486"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2585-y"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608620104"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2006.00078.x"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v6.i8.590"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/601763"},{"key":"e_1_2_10_7_2","article-title":"Contribution to ethnobotanical and floristic studies in Cameroon","volume":"641","author":"Adjanohoun J.","year":"1996","journal-title":"CSTR\/OUA Cameroon"},{"key":"e_1_2_10_8_2","first-page":"1006","article-title":"Antimicrobial effects of Chromolaena odorata on some human pathogens","volume":"3","author":"Stanley M. 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