{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:03:30Z","timestamp":1783037010419,"version":"3.54.6"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["19ZR1458100"],"award-info":[{"award-number":["19ZR1458100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Qinggan Huoxue recipe (QGHXR), a traditional Chinese medicinal formula, has a protective effect against liver fibrosis. However, the underlying mechanisms remain unclear. <jats:italic>Objective<\/jats:italic>. This study investigated the antifibrotic role of QGHXR and its underlying mechanisms. <jats:italic>Methods<\/jats:italic>. The composition of QGHXR was determined using ultra performance liquid chromatography\u2010tandem mass spectrometry (UPLC\u2010MS\/MS). Female C57BL\/6J mice were fed either a Lieber\u2013DeCarli liquid diet or pair\u2010fed control diet and intraperitoneally injected with CCl<jats:sub>4<\/jats:sub> for 8\u2009weeks (<jats:italic>n<\/jats:italic>\u2009=\u20098). In week 5, the mice were administered 100, 200, and 400\u2009mg\/kg QGHXR via oral gavage daily for 4\u2009weeks. <jats:italic>Results<\/jats:italic>. UPLC\u2010MS result showed that QGHXR contained 45 compounds including salvianolic acid A, scutellarin, baicalin, rutin, and chai saponin D. QGHXR alleviated pathological alterations in the liver. The alanine aminotransferase (ALT) level was reduced to 44.88\u2009\u00b1\u20094.39\u2009U\/L, aspartate aminotransferase (AST) to 76.25\u2009\u00b1\u20094.17\u2009U\/L, alkaline phosphatase (ALP) to 60.75\u2009\u00b1\u20095.41\u2009U\/L, and acetaldehyde to 38.54\u2009\u00b1\u20091.01\u2009U\/L compared with that of the control group (ALT 72.38\u2009\u00b1\u20095.19\u2009U\/L, AST 119.63\u2009\u00b1\u20099.82\u2009U\/L, and ALP 98.63\u2009\u00b1\u20096.71\u2009U\/L and acetaldehyde 64.86\u2009\u00b1\u20094.70\u2009U\/L). QGHXR inhibited lipid overproduction and fibrotic gene expression. The serum concentration of chemokine C\u2010X\u2010C ligand 16 (CXCL16) was reduced to 62.83\u2009\u00b1\u20096.80\u2009pg\/ml compared with that of the control group (130.91\u2009\u00b1\u200913.72\u2009pg\/mL). QGHXR downregulated CXCL16 mRNA and protein expressions. Pharmacological CXCL16 treatment reversed the QGHXR\u2010induced protective effects in ethanol plus CCl<jats:sub>4<\/jats:sub> fed mice. QGHXR reduced CXCL16 levels (91.97\u2009\u00b1\u20095.86\u2009pg\/ml) in LPS\u2010stimulated RAW264.7\u2009cells compared with that of the control group (148.68\u2009\u00b1\u20098.62\u2009pg\/ml) and inhibited toll\u2010like receptor 4 and nuclear factor\u2010kappa B phosphorylation. <jats:italic>Conclusions<\/jats:italic>. This study demonstrated that QGHXR mitigates experimental alcoholic liver fibrosis by CXCL16 inhibition, and may be considered a potential therapeutic agent for treating liver fibrosis.<\/jats:p>","DOI":"10.1155\/2023\/5642713","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T17:35:07Z","timestamp":1672767307000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Qinggan Huoxue Recipe Protects against Experimental Alcoholic Liver Fibrosis through CXCL16 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