{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:14:51Z","timestamp":1789679691171,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2020 Immune Disease Research Project of Huaiqihuang Granule","award":["CXPJJH12000003-202015"],"award-info":[{"award-number":["CXPJJH12000003-202015"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Aim and Objective<\/jats:italic>. This study was designed to explore the active compounds and significant pathways of Huaiqihuang Granule (HQHG) for treating immune thrombocytopenia (ITP) using LC\u2010MS\/MS analysis, molecular docking, and network pharmacology. <jats:italic>Materials and Methods<\/jats:italic>. Compounds of HQHG were scanned by LC\u2010MS\/MS, and the target profiles of compounds were identified based on SwissTarget Prediction. ITP target proteins were collected from various databases. Then, KEGG pathway and GO enrichment analyses were performed to explore the signaling pathways related to HQHG for ITP. The PPI and drug\u2010herbs\u2010compounds\u2010targets\u2010pathways network were constructed using Cytoscape 3.7.2. Finally, Discovery studio software was used to confirm the key targets and active compounds from HQHG. <jats:italic>Results<\/jats:italic>. A total of 187 interacting targets of 19 potentially active compounds in HQHG and 3837 ITP\u2010related targets were collected. Then, 187 intersection targets were obtained. A total of 20 key targets including EGFR, CASP3, TNF, STAT3, and ERBB2 were identified through PPI network analysis. These targets were mainly focused on the biological processes of positive regulation of protein phosphorylation, cellular response to organonitrogen compound, and cellular response to nitrogen compound. 20 possible pathways of HQHG in the treatment of ITP were identified through KEGG enrichment. EGFR, CASP3, TNF, and STAT3 are the four most important target proteins, while adenosine, caffeic acid, ferulic acid, quercetin\u20103<jats:italic>\u03b2<\/jats:italic>\u2010D\u2010glucoside, rutin, scopoletin, and tianshic acid are the most important active compounds, which were validated by molecular docking simulation. <jats:italic>Conclusion<\/jats:italic>. This study demonstrated that HQHG produced relief effects against ITP by regulating multitargets and multipathways with multicompounds. And the combined data provide novel insight of drug developing for ITP.<\/jats:p>","DOI":"10.1155\/2023\/5984361","type":"journal-article","created":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T21:20:11Z","timestamp":1673385611000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Investigation of the Active Compounds and Important Pathways of Huaiqihuang Granule for the Treatment of Immune Thrombocytopenia Using Network Pharmacology and Molecular Docking"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9175-3937","authenticated-orcid":false,"given":"Wenwen","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2761-7871","authenticated-orcid":false,"given":"Hongtao","family":"Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6414-970X","authenticated-orcid":false,"given":"Min","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9920-7252","authenticated-orcid":false,"given":"Jia","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8492-5155","authenticated-orcid":false,"given":"Wanjun","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1898-8999","authenticated-orcid":false,"family":"XiaoYang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-578336"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2017.05.010"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm6020016"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2017.12.078"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23112855"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2012.09.006"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110254"},{"key":"e_1_2_10_8_2","first-page":"122","article-title":"The effect observation of Huaiqihuang granules combined with the classical treatment for children with immune thrombocytopenia","volume":"21","author":"Yang Y.","year":"2016","journal-title":"Journal of China Pediatric Blood and Cancer"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.14053\/j.cnki.ppcr.201704006"},{"key":"e_1_2_10_10_2","first-page":"3016","article-title":"Clinical study on Huaiqihuang granules combined with recombinant human thrombopoietin in treatment of idiopathic thrombocytopenic purpura","volume":"33","author":"Yuan H.","year":"2018","journal-title":"Drugs & Clinic"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13040-020-00232-9"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz1021"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-6-13"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1037"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz981"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.5"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.27"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1074"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09234-6"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.2174\/1386207322666191022101613"},{"key":"e_1_2_10_23_2","first-page":"218","article-title":"Chemical constituents of the fruit of Licium barbarum L","volume":"7","author":"Wang Q.","year":"1998","journal-title":"Journal of Chinese Pharmaceutical Sciences"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5355"},{"key":"e_1_2_10_25_2","first-page":"110","article-title":"Analysis of chemical constitutions of Polygonatum cyrtonema dried rhizomes before and after processing with wine based on UPLC-Q-TOF-MS","volume":"27","author":"Ren H.-m.","year":"2021","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_10_26_2","first-page":"793","article-title":"Analysis of chemical constituents in the root bark of Lycium barbarum L. by HPLC-Q\/TOF MS combined with networking","volume":"43","author":"He C.","year":"2022","journal-title":"Journal of Chinese Mass Spectrometry Society"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.05.048"},{"key":"e_1_2_10_28_2","first-page":"271","article-title":"Rapid analysis and identification of the chemical constituents of wild Polygonatum kingianum Coll","volume":"48","author":"Ji K. Y.","year":"2019","journal-title":"Et Hemsl in Sichuan Aba by UPLC-Q-TOF-MS, Applied Chemical Industry"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.826902"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2010.01680.x"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-015-0251-z"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0040-1722186"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH17-03-0155"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1808217115"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.7534\/j.issn.1009-2137.2018.03.038"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2017.04.012"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1080\/08880010601166421"},{"key":"e_1_2_10_38_2","first-page":"83","volume-title":"Advances in Clinical Science","author":"Smalisz-Skrzypczyk K.","year":"2016"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7531876"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154413"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160563"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.111801"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8110557"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082276"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.860508"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1159\/000497474"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgm130"},{"key":"e_1_2_10_48_2","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.0253-2727.2015.02.004"},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119796"},{"key":"e_1_2_10_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/s10194-022-01412-0"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2023\/5984361.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2023\/5984361.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2023\/5984361","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T22:57:37Z","timestamp":1722898657000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2023\/5984361"}},"subtitle":[],"editor":[{"given":"Hu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1155\/2023\/5984361"],"URL":"https:\/\/doi.org\/10.1155\/2023\/5984361","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"2022-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5984361"}}