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Human life quality and expectancy have increased dramatically over the past 5 decades because of improvements in nutrition and antibiotic\u2019s usage fighting against infectious diseases. Yet, it was soon revealed that the microbes adapted to develop resistance to any of the drugs that were used. Recently, there is great concern that commensal bacteria from food and the gastrointestinal tract of humans and animals could act as a reservoir for antibiotic resistance genes. <jats:italic>Methodology<\/jats:italic>. This study was intended for evaluating the phenotypic antibiotic resistance\/sensitivity profiles of probiotic bacteria from human breast milk and evaluating the inhibitory effect of the probiotic bacteria against both Gram\u2010negative and Gram\u2010positive bacteria. <jats:italic>Results<\/jats:italic>. The results point out that some of the isolated bacteria were resistant to diverse antibiotics including gentamycin, imipenem, trimethoprim sulfamethoxazole, and nalidixic acid. Susceptibility profile to certain antibiotics like vancomycin, tetracycline, ofloxacin, chloramphenicol, streptomycin, rifampicin, and bacitracin was also observed. The antimicrobial qualities of cell\u2010free supernatants of some probiotic bacteria inhibited the growth of indicator bacteria. Also, antimicrobial properties of the probiotic bacteria from the present study attributed to the production of organic acid, bacterial adhesion to hydrocarbons (BATH), salt aggregation, coaggregation with pathogens, and bacteriocin production. Some isolated bacteria from human milk displayed higher hydrophobicity in addition to intrinsic probiotic properties like Gram\u2010positive classification, catalase\u2010negative activity, resistance to gastric juice (pH\u20092), and bile salt (0.3%) concentration. <jats:italic>Conclusion<\/jats:italic>. This study has added to the data of the antibiotic and antimicrobial activity of some probiotic bacteria from some samples of Pakistani women breast milk. Probiotic bacteria are usually considered to decrease gastrointestinal tract diseases by adhering to the gut epithelial and reducing population of pathogens and in the case of <jats:italic>Streptococcus lactarius<\/jats:italic> MB622 and <jats:italic>Streptococcus salivarius<\/jats:italic> MB620 in terms of hydrophobicity and exclusion of indicator pathogenic strains.<\/jats:p>","DOI":"10.1155\/2023\/6399699","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:05:05Z","timestamp":1687219505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Unveiling the Antibiotic Susceptibility and Antimicrobial Potential of Bacteria from Human Breast Milk of Pakistani Women: An Exploratory Study"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7575-5377","authenticated-orcid":false,"given":"Ayesha","family":"Saeed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hina","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azra","family":"Yasmin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehreen","family":"Baig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abd","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5009-483X","authenticated-orcid":false,"given":"Abeer","family":"Kazmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Arslan","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghadeer M.","family":"Albadrani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5624-9145","authenticated-orcid":false,"given":"Fatma M.","family":"El-Demerdash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monaza","family":"Bibi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4341-2713","authenticated-orcid":false,"given":"Mohamed M.","family":"Abdel-Daim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2319-2175","authenticated-orcid":false,"given":"Iftikhar","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadam","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-40422-2_5"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000346"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.3920\/BM2011.0016"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781003189183-5"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2023.156232"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2021.100993"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2021.10.020"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145203"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/8726320"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9100688"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2021.6490"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-021-01703-7"},{"key":"e_1_2_12_13_2","first-page":"35","article-title":"Evaluation of antibiotic self-medication pattern amongst undergraduate students of Ahmadu Bello University (Main Campus) Zaria","volume":"2","author":"Olayemi O. 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