{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:32:58Z","timestamp":1791387178347,"version":"4.3.3"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":103,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The perennial plant <jats:italic>Hypericum perforatum<\/jats:italic> is widely distributed around the world. It has been used for many years in conventional medicine to treat a variety of illnesses, including stress, mild to moderate depression, and minor injuries. This study examined the antimicrobial activity of the <jats:italic>H. perforatum<\/jats:italic> total extract and its fractions (n\u2010hexane, ethyl acetate, chloroform, and aqueous) against multi\u2010drug\u2010resistant (MDR) isolates that were gathered from clinical samples, including methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA), <jats:italic>Enterococcus faecalis<\/jats:italic>, <jats:italic>Escherichia coli<\/jats:italic>, and <jats:italic>Klebsiella pneumonia<\/jats:italic>. <jats:italic>Materials and Methods<\/jats:italic>. Aerial parts of <jats:italic>H. perforatum<\/jats:italic> were collected and extracted using various solvents and were tested versus different isolated bacterial species. The inhibition zone of tested extracts was detected using an agar diffusion assay, and MICs were measured. Phytochemical analysis of promising <jats:italic>H. perforatum<\/jats:italic> extract was done using LC\u2010ESI\u2010MS\/MS. Ultrastructure examination for the most altered bacteria used transmission electron microscopy. Antioxidant assays were done using DPPH and ABTS scavenging capacity methods. Cytotoxicity was reported versus Vero cells. <jats:italic>Results<\/jats:italic>. Different extracts of <jats:italic>H. perforatum<\/jats:italic> showed promising antibacterial activity against the pathogens. While the subfractions of the total extract were observed to show lesser inhibition zones and higher MIC values than the total extract of <jats:italic>H. perforatum<\/jats:italic> against MDR strains, the total extract of <jats:italic>H. perforatum<\/jats:italic> demonstrated the most potent antimicrobial action with an inhibition zone range of 17.9\u201027.9\u2009mm. MDR\u2010<jats:italic>K. pneumoniae<\/jats:italic> was discovered to be the most susceptible strain, which is consistent with the antibacterial inhibitory action of <jats:italic>H. perforatum<\/jats:italic> whole extract. Additionally, after treatment at the minimum inhibitory concentration (MIC 3.9\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml), the transmission electron microscope showed alterations in the ultrastructure of the <jats:italic>K. pneumoniae<\/jats:italic> cells. Methanol extract from <jats:italic>H. perforatum<\/jats:italic> has a CC<jats:sub>50<\/jats:sub> value of 976.75\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml. <jats:italic>Conclusion<\/jats:italic>. Future inhibitors that target MDR strains may be revealed by these findings. Additionally, the extracts that were put to the test demonstrated strong antioxidant effects as shown by DPPH or ABTS radical\u2010scavenging assays.<\/jats:p>","DOI":"10.1155\/2023\/6934398","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T18:35:09Z","timestamp":1681497309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["<i>In Vitro<\/i> Antibacterial and Phytochemical Screening of <i>Hypericum perforatum<\/i> Extract as Potential Antimicrobial Agents against Multi\u2010Drug\u2010Resistant (MDR) Strains of Clinical Origin"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9216-1052","authenticated-orcid":false,"given":"Momen M.","family":"Sherif","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussien H.","family":"Elshikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2140-0655","authenticated-orcid":false,"given":"Marwa M.","family":"Abdel-Aziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0862-7628","authenticated-orcid":false,"given":"Mahmoud M.","family":"Elaasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7225-8658","authenticated-orcid":false,"given":"Mohammed","family":"Yosri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,14]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1159366"},{"key":"e_1_2_9_2_2","first-page":"311","volume-title":"Plant Extracts: Role in Agriculture, Health Effects and Medical Applications","author":"Tayel A. 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