{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:55:28Z","timestamp":1747191328858,"version":"3.40.5"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:00:00Z","timestamp":1687478400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2023,6,23]]},"abstract":"<jats:p>Leptospirosis is a zoonosis with ubiquitous distribution caused by spirochetes belonging to the genus Leptospira sp., endemic mainly in tropical and subtropical regions of the world and capable of infecting domestic animals, free-living animals, and humans. Although well documented in terrestrial animals and humans, little information is available on its distribution and impact on marine animals. There are few studies assessing cetaceans\u2019 health status, and even scarcer are those focused on leptospirosis research. In this context, considering the One Health approach, the present study aimed to investigate the occurrence of pathogenic Leptospira sp. in cetaceans on the Brazilian coast. Kidneys of 142 cetaceans belonging to 19 species were collected. DNA was extracted, and the diagnosis was performed by LipL32-polymerase chain reaction. Genetic characterization was conducted based on secY gene sequencing. Pathogenic Leptospira sp. DNA was detected in 14.8% (21\/142) of the tested cetaceans, with coastal species presenting a significantly higher frequency (p-value\u2009=\u20090.03) of infected individuals (25%, 17\/68) than oceanic species (7.5%, 4\/53). It was possible to amplify and sequence three strains (one for Sotalia guianensis, one for Stenella clymene, and one for Pontoporia blainvillei), all of them identified as Leptospira interrogans, with high similarity with sequences from Icterohaemorrhagiae serogroup. Phylogenetic analysis revealed sequences from the present study grouped in species-specific unique clusters but very close to pinnipeds in the same area, evidencing the presence of two distinct haplotypes circulating on marine mammals in the region. We could demonstrate that cetaceans can act as carriers of pathogenic leptospires. Moreover, the proximity with anthropogenic areas could play an important role in leptospirosis\u2019 dynamics of transmission in a One Health context.<\/jats:p>","DOI":"10.1155\/2023\/7041089","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T20:37:41Z","timestamp":1687552661000},"page":"1-8","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Detection of Pathogenic Leptospira sp. in Cetaceans from the Brazilian Coast"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5738-0022","authenticated-orcid":true,"given":"Felipe D\u2019Azeredo","family":"Torres","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute, Fluminense Federal University, Niter\u00f3i, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5592-3687","authenticated-orcid":true,"given":"Ana Luiza dos Santos Baptista","family":"Borges","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute, Fluminense Federal University, Niter\u00f3i, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9939-7807","authenticated-orcid":true,"given":"Pedro Volkmer de","family":"Castilho","sequence":"additional","affiliation":[{"name":"Laboratory of Zoology, University of the State of Santa Catarina, Laguna, Santa Catarina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8048-6551","authenticated-orcid":true,"given":"Cristiane","family":"Kolesnikovas","sequence":"additional","affiliation":[{"name":"R3 Animal Association, Florian\u00f3polis 88061-500, Santa Catarina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6158-6963","authenticated-orcid":true,"given":"Camila","family":"Domit","sequence":"additional","affiliation":[{"name":"Laboratory of Ecology and Conservation, Universidade Federal do Paran\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4217-7088","authenticated-orcid":true,"given":"Jonathas","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Beach Monitoring Project of Sergipe-Alagoas Basin, Vichi Management Projects, Aracaju, Sergipe, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8887-9969","authenticated-orcid":true,"given":"Waltyane Alves Gomes","family":"Bonfim","sequence":"additional","affiliation":[{"name":"Biota Conservation Institute, Macei\u00f3, Alagoas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6765-0559","authenticated-orcid":true,"given":"Vitor Luz","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Association for Research and Preservation of Aquatic Ecosystems, Caucaia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Beatriz","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Argonauta Institute for Coastal and Marine Conservation, Ubatuba, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Lanes","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Biopesca Institute, Praia Grande, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8083-2840","authenticated-orcid":true,"given":"Filipe Anibal","family":"Carvalho-Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-3130","authenticated-orcid":true,"given":"Maria Isabel Nogueira","family":"Di Azevedo","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute, Fluminense Federal University, Niter\u00f3i, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2434-8728","authenticated-orcid":true,"given":"Walter","family":"Lilenbaum","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute, Fluminense Federal University, Niter\u00f3i, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"2","key":"1","doi-asserted-by":"crossref","first-page":"134","DOI":"10.5670\/oceanog.2006.77","article-title":"Marine mammals as sentinel species for oceans and human health","volume":"19","author":"G. 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