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Here, a PEDV CH\/Yinchuan\/2021 strain was isolated in a CV777-vaccinated sow farm which experienced a large-scale PEDV invasion in Yinchuan, China, in 2021. Our results demonstrated that the CH\/Yinchuan\/2021 isolate could efficiently propagate in Vero cells, and its proliferation ability was weaker than that of CV777 at 10 passages (P10). Phylogenetic analysis of the S gene revealed that CH\/Yinchuan\/2021 was clustered into subgroup GIIa, forming an independent branch with 2020-2021 isolates in China. Moreover, GII was obviously allocated into four clades, showing regional and temporal differences in PEDV global isolates. Notably, CH\/Yinchuan\/2021 was analyzed as a recombinant originated from an American isolate and a Chinese isolate, with a big recombinant region spanning ORF1a and S1. Importantly, we found that CH\/Yinchuan\/2021 harbored multiple mutations relative to CV777 in neutralizing epitopes (S10, S1A, COE, and SS6). Homology modelling showed that these amino acid differences in S protein occur on the surface of its structure, especially the insertion and deletion of multiple consecutive residues at the S10 epitope. In addition, cross-neutralization analysis confirmed that the differences in the S protein of CH\/Yinchuan\/2021 changed its antigenicity compared with the CV777 strain, resulting in a different neutralization profile. Animal pathogenicity test showed that CH\/Yinchuan\/2021 caused PEDV-typified symptoms and 100% mortality in 3-day-old piglets. These data will provide valuable information to understand the epidemiology, molecular characteristics, evolution, and antigenicity of PEDV circulating in China.<\/jats:p>","DOI":"10.1155\/2023\/7082352","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T15:05:15Z","timestamp":1678201515000},"page":"1-17","source":"Crossref","is-referenced-by-count":5,"title":["A Porcine Epidemic Diarrhea Virus Isolated from a Sow Farm Vaccinated with CV777 Strain in Yinchuan, China: Characterization, Antigenicity, and Pathogenicity"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7921-8111","authenticated-orcid":true,"given":"Jianlin","family":"Lei","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Shaanxi, Yangling, China"},{"name":"College of Agriculture and Forestry Science and Technology, Longdong University, Qingyang, 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