{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:44:42Z","timestamp":1789685082183,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:00:00Z","timestamp":1675123200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2023,1,31]]},"abstract":"<jats:p>Purpose. Gastric cancer (GC) is one of the most common malignant tumors and also one of the most deadly tumors. In recent years, studies have shown that propofol can inhibit the proliferation and metastasis of many tumor cells. In the present study, we aimed to investigate the underlying mechanism of propofol inhibition of the growth and invasion of GC cells. Methods. Human gastric cancer cell line SGC-7901 and human normal gastric epithelial cell GES-1 were cultured in high-glucose Dulbecco\u2019s Modified Eagle\u2019s Medium (DMEM) supplemented with 10% fetal bovine serum at 37\u00b0C with 5% CO2. Propofol of different concentrations (0, 2, 5, and 10\u2009\u03bcg\/mL) was used to treat SGC-7901, and miR-493-3p inhibitor was transfected into SGC-7901. The cell proliferation of SGC-7901 was analyzed by MTT as well as colony formation assay. The qRT-PCR was used to assess the expression of mRNA for key genes. We examined the protein expression of DKK1 and relative markers with western blot. Putative binding places of miR-493-3p on the 3<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mo>\u2032<\/a:mo>\n                     <\/a:math>\n                  <\/jats:inline-formula>-untranslated area of DKK1 were predicted using bioinformatics and dual-luciferase method. Results. Propofol prohibited phenotypic features of GC, according to our findings. Furthermore, research into the underlying mechanisms of propofol\u2019s suppressive effects in GC cell proved that propofol therapy improved the degrees of expression of the potential tumor suppressor miR-493-3p. The inhibiting properties of propofol on GC cell development, migration, and invasion were abolished when propofol-induced miR493-3p was silenced with anti-miR-493-3p. We also found that this drug reversed epithelial-mesenchymal transformation in SGC-7901 cells via inducing miR-493-3p. Propofol-induced miR-493-3p decreases GC cell development via targeting DKK1 and hence inhibits Wnt\/\u03b2-catenin signaling, according to these findings. Conclusion. Propofol-induced miR-493-3p decreased GC cell development via targeting DKK1 and hence inhibited Wnt\/\u03b2-catenin signaling, according to these findings.<\/jats:p>","DOI":"10.1155\/2023\/7698706","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T15:05:41Z","timestamp":1675177541000},"page":"1-8","source":"Crossref","is-referenced-by-count":14,"title":["Propofol-Induced miR-493-3p Inhibits Growth and Invasion of Gastric Cancer through Suppression of DKK1-Mediated Wnt\/\u03b2-Catenin Signaling Activation"],"prefix":"10.1155","volume":"2023","author":[{"given":"Kaishuai","family":"Zhan","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Shandong First Medical University, Jinan, 250031 Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Shandong First Medical University, Jinan, 250031 Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Shandong First Medical University, Jinan, 250031 Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianshu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Shandong First Medical University, Jinan, 250031 Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1376-4539","authenticated-orcid":true,"given":"Shoutang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Shandong First Medical University, Jinan, 250031 Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111991"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i33.5485"},{"issue":"13","key":"4","first-page":"1722","article-title":"Propofol inhibits invasion and enhances paclitaxel-induced apoptosis in ovarian cancer cells through the suppression of the transcription factor slug","volume":"17","author":"P. 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