{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T02:51:55Z","timestamp":1786243915767,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":155,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Despite the widespread introduction of primary and secondary preventative measures, death rates for cervical cancer are still significantly high among females, especially in developing countries. Pap cytology and human papillomavirus\u2010based screening often lead to unnecessary additional testing. The aim of this study is to analyze diagnostic accuracy of p16<jats:sup>INK4a<\/jats:sup>\/Ki\u201067 dual immunostaining (DS) in cervical smear for identifying high\u2010grade cervical intraepithelial neoplasia (CIN2+). <jats:italic>Materials and Methods<\/jats:italic>. We studied the diagnostic performance of p16<jats:sup>INK4a<\/jats:sup>\/Ki\u201067 DS in cervical smear of those women, who enrolled in cervical cancer screening due to abnormal previous screening results and compared it with Pap test results in identifying CIN2+. The reference standard was histopathology results. p16<jats:sup>INK4a<\/jats:sup>\/Ki\u201067 DS and Pap test results for 162 women and histopathology results for 29 women were available, respectively. <jats:italic>Results<\/jats:italic>. In our study, sensitivity, specificity, positive predictive value, and negative predictive value of p16<jats:sup>INK4a<\/jats:sup>\/Ki\u201067 DS, irrespective of the morphology of stained cells to detect CIN2+ were 100%, 89%, 85%, and 100% (<jats:italic>p<\/jats:italic> &lt; 0.01), respectively. The diagnostic accuracy of p16<jats:sup>INK4a<\/jats:sup>\/Ki\u201067 DS is superior to that of existing cervical screening tests in the detection of CIN2+. <jats:italic>Conclusion<\/jats:italic>. The findings of cervical cancer screening based on Pap cytology highlight the importance of assessing the cost\u2010effectiveness of integrating p16<jats:sup>INK4a<\/jats:sup>\/Ki\u201067 biomarkers in cervical cancer cytology. Furthermore, these findings emphasize the need to enhance support for preventive programs for cervical cancer in Georgia.<\/jats:p>","DOI":"10.1155\/2023\/7988323","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T18:20:09Z","timestamp":1685989209000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic Accuracy of p16<sup>INK4a<\/sup>\/Ki\u201067 Dual Immunostaining for Detection of High\u2010Grade Cervical Intraepithelial Neoplasia in Women Involved in Cervical Cancer Screening in Georgia"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7300-4107","authenticated-orcid":false,"given":"Sopio","family":"Kakaliashvili-Dzagnidze","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Khardzeishvili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergo","family":"Tabagari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-46334-6_5"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006250-197803000-00028"},{"key":"e_1_2_9_4_2","first-page":"301","article-title":"Cervical intraepithelial neoplasia","volume":"8","author":"Richart R. M.","year":"1973","journal-title":"Pathology Annual"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2009.05.046"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2114"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/0049475518798532"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.9.690"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(97)00177-0"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61416-0"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pvr.2015.06.006"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21628"},{"key":"e_1_2_9_13_2","unstructured":"World Health Organization Cervical cancer country profiles 2021 https:\/\/www.who.int\/publications\/m\/item\/cervical-cancer-country-profiles."},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.a1284"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.291.17.2100"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000151991.09124.bb"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30524"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.02.033"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-419X(98)00017-1"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.9.4350"},{"key":"e_1_2_9_21_2","first-page":"4620","article-title":"E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway","volume":"56","author":"Boyer S. N.","year":"1996","journal-title":"Cancer Research"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(02)00462-8"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26017"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canep.2010.12.006"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1159\/000477556"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.5858\/2001-125-0134-RCATFP"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10109"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.LGT200570"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.2478\/raon-2021-0043"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt235"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.80"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20140"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24325"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990825)87:4<184::AID-CNCR4>3.0.CO;2-#"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.5858\/134.3.331"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2021.36.e164"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.14744\/nci.2018.93270"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2018.4270"}],"container-title":["Analytical Cellular Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2023\/7988323.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2023\/7988323.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2023\/7988323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T18:43:46Z","timestamp":1733942626000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2023\/7988323"}},"subtitle":[],"editor":[{"given":"Giovanni","family":"Tuccari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1155\/2023\/7988323"],"URL":"https:\/\/doi.org\/10.1155\/2023\/7988323","archive":["Portico"],"relation":{},"ISSN":["2210-7177","2210-7185"],"issn-type":[{"value":"2210-7177","type":"print"},{"value":"2210-7185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"2023-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7988323"}}