{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:39:16Z","timestamp":1783427956448,"version":"3.54.6"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:00:00Z","timestamp":1688083200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005274","name":"North-West University","doi-asserted-by":"publisher","award":["UID91990"],"award-info":[{"award-number":["UID91990"]}],"id":[{"id":"10.13039\/501100005274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005274","name":"North-West University","doi-asserted-by":"publisher","award":["UID86625"],"award-info":[{"award-number":["UID86625"]}],"id":[{"id":"10.13039\/501100005274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["UID91990"],"award-info":[{"award-number":["UID91990"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["UID86625"],"award-info":[{"award-number":["UID86625"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2023,6,30]]},"abstract":"<jats:p>Rhizobial nitrogen-fixing bacteria are the main inhabitants of the root nodules of legume plants. Studying the bacterial community of legume nodules is important in understanding plant growth and nutrient requirements. Culture-based technique was used to examine the bacterial community of these underground organs from Vigna subterranea L. Verdc (Bambara nut), an underutilized legume in Africa, for plant growth-promoting traits. In this study, Bambara nuts were planted to trap root-nodule bacteria, and the bacteria were morphologically, biochemically, and molecularly characterized. Five selected isolates were screened in vitro for their plant growth-promoting traits and exhibited differences in their phenotypic traits. The polymerase chain reaction (PCR) products were subjected to partial 16S rRNA gene sequencing for phylogenetic analysis. Based on 16S rRNA gene sequence, the isolates were identified as BA1 (Stenotrophomonas maltophilia), BA2 (Chryseobacterium sp.), BA3 (Pseudomonas alcaligenes), BA4 (Pseudomonas plecoglossicida), and BA5 (Pseudomonas hibiscicola). Results showed that four of the five isolates could produce IAA. The capability to solubilize phosphate in Pikovskaya\u2019s agar plates was positively shown by four isolates (BA2, BA3, BA4, and BA5). Three isolates could produce hydrogen cyanide while isolates BA1, BA3, BA4, and BA5 were found to have ammonia-production traits. The results suggest that these plant growth-promoting isolates can be used as inoculants for plant growth and productivity.<\/jats:p>","DOI":"10.1155\/2023\/8218721","type":"journal-article","created":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T08:35:08Z","timestamp":1688200508000},"page":"1-8","source":"Crossref","is-referenced-by-count":5,"title":["Bambara Nut Root-Nodules Bacteria from a Semi-Arid Region of South Africa and Their Plant Growth-Promoting Traits"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3220-1873","authenticated-orcid":true,"given":"Ayansina Segun","family":"Ayangbenro","sequence":"first","affiliation":[{"name":"Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohomud Rashid","family":"Adem","sequence":"additional","affiliation":[{"name":"Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4344-1909","authenticated-orcid":true,"given":"Olubukola Oluranti","family":"Babalola","sequence":"additional","affiliation":[{"name":"Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/932947"},{"key":"2","first-page":"60","article-title":"Bambara groundnut: an under-utilized nut in Africa","volume":"1","author":"O. Bamshaiye","year":"2011","journal-title":"Advances in agricultural biotechnology"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/472129"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3803(20010401)45:2<94::aid-food94>3.0.co;2-e"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1108\/00346650610642151"},{"issue":"2","key":"6","first-page":"65","article-title":"Isolation and screening of indigenous Bambara groundnut (Vigna subterranea) nodulating bacteria for their tolerance to some environmental stresses","volume":"3","author":"N. N. Laurette","year":"2015","journal-title":"American Journal of Microbiological Research"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-021-05247-7"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.825377"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414592112"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1080\/07352680701572966"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01099-22"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2018.e00833"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-86977-7"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-022-02827-3"},{"key":"15","article-title":"Microbiology: a laboratory manual","author":"J. G. Cappuccino","year":"2005","journal-title":"Pearson\/Benjamin Cummings San Francisco"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1948.tb07118.x"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.2.535-538.1991"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13383.x"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.697-703.1991"},{"key":"20","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume-title":"Nucleic Acids Symposium Series","author":"T. A. Hall","year":"1999"},{"issue":"21","key":"21","first-page":"132","article-title":"Evolution of protein molecules","volume":"3","author":"T. H. Jukes","year":"1969","journal-title":"Mammalian Protein Metabolism"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213199109"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.crbiot.2022.01.003"},{"key":"25","first-page":"725","article-title":"The root-nodule bacteria","volume":"46","author":"M. W. Beijerinck","year":"1888","journal-title":"Botanische Zeitung"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2011.01063.x"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-007-0211-3"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1094\/pbiomes-12-16-0019-rvw"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2007.00424.x"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1128\/aem.71.9.4951-4959.2005"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-017-2364-9"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01785"},{"issue":"2","key":"33","first-page":"108","article-title":"Plant growth promoting rhizobacteria (PGPR): a review","volume":"5","author":"C. K. Jha","year":"2015","journal-title":"Journal of Agricultural Research and Development"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2006.04.001"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2013.08.009"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2013.05.001"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-010-0347-0"},{"issue":"3","key":"38","first-page":"443","article-title":"Isolation of plant growth-promoting strains of Bradyrhizobium (Arachis) sp. with biocontrol potential against Macrophomina phaseolina causing charcoal rot of peanut","volume":"84","author":"V. Deshwal","year":"2003","journal-title":"Current Science"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1080\/17429145.2020.1752833"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2015.12.003"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3846(200102)21:1<65::aid-abio65>3.0.co;2-#"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1016\/s0944-5013(00)80047-6"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2015.04.002"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2016.07.002"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcl027"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-013-0095-y"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2004.00116.x"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2005.12.002"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2004.187.196"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1186\/s43141-020-00090-2"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-013-1488-9"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-009-0168-x"},{"issue":"25","key":"53","first-page":"3794","article-title":"Performance of phosphate solubilizing bacteria for improving growth and yield of sunflower (Helianthus annuus L.) in the presence of phosphorus fertilizer","volume":"9","author":"Z. Ekin","year":"2010","journal-title":"African Journal of Biotechnology"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7110519"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2007.02.005"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj1999.6361670x"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1080\/17429145.2013.768360"}],"container-title":["International Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2023\/8218721.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2023\/8218721.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2023\/8218721.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T08:35:14Z","timestamp":1688200514000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijmicro\/2023\/8218721\/"}},"subtitle":[],"editor":[{"given":"David M.","family":"Iovannisci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,6,30]]},"references-count":57,"alternative-id":["8218721","8218721"],"URL":"https:\/\/doi.org\/10.1155\/2023\/8218721","relation":{},"ISSN":["1687-9198","1687-918X"],"issn-type":[{"value":"1687-9198","type":"electronic"},{"value":"1687-918X","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6,30]]}}}