{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T19:29:49Z","timestamp":1784057389903,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":152,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007119","name":"Yasuj University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007119","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Nowadays, due to various inherent properties, graphene\u2010based nanoparticles are widely used in drug delivery research. On the other hand, folate receptors are highly expressed on the surface of human tumor cells. In this work, to enhance the 5\u2010fluorouracil (5FU) and curcumin (Cur) effects on colon cancer, we constructed a folic acid\u2010 (FA\u2010) modified codelivery carrier based on graphene nanoparticles (GO\u2010Alb\u2010Cur\u2010FA\u20105FU). <jats:italic>Materials and Methods<\/jats:italic>. The HUVEC and HT\u201029 were selected for evaluating the antitumor effect of the prepared nanocarriers. The structure of nanocarriers was characterized by FTIR spectroscopy, X\u2010ray diffraction analysis, TEM microscopy, and a DLS analyzer. The efficiency of the prepared carrier was evaluated by fluorescence microscopy using Annexin V and the PI kit. The cytotoxicity of the carrier\u2019s component individually and the efficacy of the drug carrier GO\u2010Alb\u2010Cur\u2010FA\u20105FU were assessed by MTT. <jats:italic>Results<\/jats:italic>. The results of the pharmacological tests indicated that the new nanoparticles cause increased apparent toxicity in HT\u201029 cells. The apoptosis rate of the HT\u201029 and HUVEC cells treated with IC50 values of GO\u2010Alb\u2010Cur\u2010FA\u20105FU for 48\u2009h was higher than the cells treated with IC50 values of 5FU and Cur individually, which indicated the greater inhibitory efficacy of GO\u2010Alb\u2010Cur\u2010FA\u20105FU than free drugs. <jats:italic>Conclusion<\/jats:italic>. The designed GO\u2010Alb\u2010CUR\u2010FA\u20105FU delivery system can be applied for targeting colon cancer cells and can be severe as a potential candidate for future drug development.<\/jats:p>","DOI":"10.1155\/2023\/8334102","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T20:35:12Z","timestamp":1685738112000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Folic Acid\u2010Functionalized Albumin\/Graphene Oxide Nanocomposite to Simultaneously Deliver Curcumin and 5\u2010Fluorouracil into Human Colorectal Cancer Cells: An <i>In Vitro<\/i> Study"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8799-173X","authenticated-orcid":false,"given":"Hassan","family":"Bardania","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farajollah","family":"Jafari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzieh","family":"Baneshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Mahmoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam Tajali","family":"Ardakani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farshad","family":"Safari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7240-6614","authenticated-orcid":false,"given":"Mehrzad Jafari","family":"Barmak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrpr.2017.07.001"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00341.x"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/M20-0068"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.21147\/j.issn.1000-9604.2021.01.01"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115786"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.05.018"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-3657"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/0885328220949367"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/0885328220976331"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-008-0260-0"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16064567"},{"key":"e_1_2_9_12_2","article-title":"Using curcumin to prevent structural impairments of testicles in rats induced by sodium metabisulfite","volume":"16","author":"Mahmoudi R.","year":"2017","journal-title":"EXCLI Journal"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160922094"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2021.117710"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40204-022-00184-9"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2020.29.10.586"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2021.12.009"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2021.112091"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-020-05556-7"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40204-018-0104-3"},{"key":"e_1_2_9_21_2","first-page":"955","article-title":"Synthesis, characterization and cytotoxic evaluation of graphene oxide nanosheets: in vitro liver cancer model","volume":"18","author":"Loutfy S. 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