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However, cardiotoxicity\u2019s side effect limits its clinical applicability. In the present study, Gene Expression Omnibus (GEO) datasets were applied to reanalyze differentially expressed genes (DEGs) and construct weighted correlation network analysis (WGCNA) modules of doxorubicin-induced cardiotoxicity in wild-type mice. Several other bioinformatics analyses were performed to pick out the hub gene, and then the correlation between the hub gene and immune infiltration was evaluated. In total, 120 DEGs were discovered in a mouse model of doxorubicin-induced cardiotoxicity, and PF-04217903, propranolol, azithromycin, etc. were found to be potential drugs against this pathological condition. Among all the DEGs, 14 were further screened out by WGCNA modules, of which Limd1 was upregulated and finally regarded as the hub gene after being validated in other GEO datasets. Limd1 was upregulated in the peripheral blood mononuclear cell (PBMC) of the rat model, and the area under curve (AUC) of the receiver operating characteristic curve (ROC) in diagnosing cardiotoxicity was 0.847. The GSEA and PPI networks revealed a potential immunocyte regulatory role of Limd1 in cardiotoxicity. The proportion of \u201cdendritic cells activated\u201d in the heart was significantly elevated, while \u201cmacrophage M1\u201d and \u201cmonocytes\u201d declined after in vivo doxorubicin application. Finally, Limd1 expression was significantly positively correlated with \u201cdendritic cells activation\u2019 and negatively correlated with \u201cmonocytes\u201d and \u201cmacrophages M1\u2019. In summary, our results suggested that limd1 is a valuable biomarker and a potential inflammation regulator in doxorubicin-induced cardiotoxicity.<\/jats:p>","DOI":"10.1155\/2023\/8347759","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T03:50:06Z","timestamp":1679629806000},"page":"1-23","source":"Crossref","is-referenced-by-count":5,"title":["Upregulation of Biomarker Limd1 Was Correlated with Immune Infiltration in Doxorubicin-Related Cardiotoxicity"],"prefix":"10.1155","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8655-4017","authenticated-orcid":true,"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1186-4312","authenticated-orcid":true,"given":"Chunshu","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0299-8527","authenticated-orcid":true,"given":"Zhenjun","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1285-8584","authenticated-orcid":true,"given":"Yangyang","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8289-5113","authenticated-orcid":true,"given":"Wenjie","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4484-0979","authenticated-orcid":true,"given":"Mingming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7780-0845","authenticated-orcid":true,"given":"Pengfei","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1438-7907","authenticated-orcid":true,"given":"Abdlay","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1772-3278","authenticated-orcid":true,"given":"Genshan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0936-2644","authenticated-orcid":true,"given":"Yongjun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, 87 Hunan Road, Nanjing, Jiangsu 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101394"},{"key":"2","volume-title":"Doxorubicin, StatPearls, StatPearls Publishing Copyright \u00a9 2021","author":"K. 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