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However, the potential material basis and molecular mechanism of Shudage\u20104 in attenuating stress\u2010induced gastric ulcers remain unclear. This study aimed to first explore the potential material basis and molecular mechanism of Shudage\u20104 in attenuating gastric ulcers in rats. The chemical constituents and transitional components in the blood of Shudage\u20104 were identified by ultra\u2010performance liquid chromatography time\u2010of\u2010flight mass spectrometry (UPLC\u2010TOF\u2010MS). The rat gastric ulcer model was induced by water immersion restraint stress (WIRS). The ulcer damage to gastric tissue was measured at the gross anatomical level and pathological level by hematoxylin\u2010eosin (HE) staining of gastric tissue. RNA sequencing of gastric tissue and plasma metabolomics were performed to analyze the mechanism of Shudage\u20104 against gastric ulcers. A Pearson correlation analysis was performed to explore the association between serum metabolites and gene expression of gastric tissue. A total of 30 chemical constituents were identified in Shudage\u20104 by UPLC\u2010TOF\u2010MS. Among 30 constituents, 13 transitional components in the blood were considered as the potential material basis. Shudage\u20104 treatment had a significant effect on WIRS\u2010induced gastric ulcers in rats. HE staining of gastric tissue illustrated that WIRS\u2010induced ulcer damage was suppressed by Shudage\u20104 treatment. RNA sequencing of gastric tissue showed that 282 reversed expression genes in gastric tissue were related to Shudage\u20104 treatment, and gene set enrichment analysis revealed that Shudage\u20104 treatment significantly inhibited gene set expression related to reactive oxygen species (ROS), which was also validated by detecting rat gastric tissue MDA, GSH, SOD, GSH\u2010Px, and CAT activities. The plasma metabolomic data demonstrated that 23 significantly differential metabolites were closely associated with the Shudage\u20104 treatment. The further multiomics joint analysis found that significantly upregulated 5 plasma metabolites in Shudage\u20104\u2010treated rats compared to model rats were negatively correlated with gene set expression related to ROS in gastric tissue. Shudage\u20104 alleviated WIRS\u2010induced gastric ulcers by inhibiting ROS generation, which was achieved by regulating plasma metabolites level.<\/jats:p>","DOI":"10.1155\/2023\/8998368","type":"journal-article","created":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T20:05:13Z","timestamp":1686945913000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Systematic Exploration of the Potential Material Basis and Molecular Mechanism of the Mongolian Medicine Shudage\u20104 in Attenuating Stress\u2010Induced Gastric Ulcer in Rat"],"prefix":"10.1155","volume":"2023","author":[{"given":"Xin","family":"Jia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuexuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2634-1116","authenticated-orcid":false,"given":"Tianlong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5942-6239","authenticated-orcid":false,"given":"Yu","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,16]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/08923973.2017.1379088"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i45.17020"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.11.059"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051579"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.1c01801"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2008.09.015"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1985.tb01116.x"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5149"},{"key":"e_1_2_11_9_2","first-page":"1255","article-title":"Guilty as charged: bugs and drugs in gastric ulcer","volume":"92","author":"Sontag S. 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