{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T07:04:14Z","timestamp":1781939054765,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["FOOD66310011"],"award-info":[{"award-number":["FOOD66310011"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["N42A650553"],"award-info":[{"award-number":["N42A650553"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["FOOD66310011"],"award-info":[{"award-number":["FOOD66310011"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["N42A650553"],"award-info":[{"award-number":["N42A650553"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Second Century Fund","award":["FOOD66310011"],"award-info":[{"award-number":["FOOD66310011"]}]},{"name":"Second Century Fund","award":["N42A650553"],"award-info":[{"award-number":["N42A650553"]}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["FOOD66310011"],"award-info":[{"award-number":["FOOD66310011"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A650553"],"award-info":[{"award-number":["N42A650553"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2023,11,28]]},"abstract":"<jats:p>Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, causes severe neurological disorders and acute egg drop syndrome in ducks. Currently, several clusters of DTMUV, including clusters 1, 2, and 3, have been identified and caused outbreaks in Asia. However, most of the DTMUV pathogenesis evaluation has mainly focused on cluster 2, while limited information is available on the pathogenesis of other DTMUV clusters, particularly cluster 1. In this study, the pathogenesis of a cluster 1 DTMUV was investigated in Cherry Valley ducks and compared to our previously reported cluster 2.1 DTMUV. Our results demonstrated that cluster 1 DTMUV was generally less pathogenic than cluster 2.1 DTMUV in ducks as evidenced by slower body weight loss, lower morbidity and mortality rates, and milder pathological changes. Concordantly, delayed viremia, reduced viral loads in blood and tissues, and shorter shedding period with lower viral loads were also observed in cluster 1 DTMUV inoculated ducks compared with those reported in cluster 2.1 DTMUV. In addition, we also found that cluster 1 DTMUV exhibited significant antigenic difference compared to cluster 2.1 DTMUV. Altogether, our findings suggest distinct pathogenicity and antigenicity between cluster 1 and 2.1 DTMUVs in ducks, highlighting the potential association between DTMUV genotype and pathogenicity\/disease severity. This study enhances our understanding of DTMUV pathogenesis in ducks and provides useful information for the design and development of effective DTMUV vaccines.<\/jats:p>","DOI":"10.1155\/2023\/9239953","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:23:25Z","timestamp":1701217405000},"page":"1-16","source":"Crossref","is-referenced-by-count":7,"title":["Pathogenesis of Cluster 1 Duck Tembusu Virus in Ducks Reveals the Impact of Viral Genotype on Pathogenicity and Disease Severity"],"prefix":"10.1155","volume":"2023","author":[{"given":"Wikanda","family":"Tunterak","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanana","family":"Rungprasert","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwarak","family":"Wannaratana","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-Ok, Chonburi, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichapat","family":"Yurayart","sequence":"additional","affiliation":[{"name":"Animal Vector-Borne Disease Research Unit, Veterinary Parasitology Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duangduean","family":"Prakairungnamthip","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patchareeporn","family":"Ninvilai","sequence":"additional","affiliation":[{"name":"Avian Veterinary Services, CPF (Thailand) Public Company Limited, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benchaphorn","family":"Limcharoen","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teerawut","family":"Nedumpun","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8603-7039","authenticated-orcid":true,"given":"Rodolphe","family":"Hamel","sequence":"additional","affiliation":[{"name":"MIVEGEC, Univ Montpellier, CNRS, IRD, Montpellier, France"},{"name":"Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, Thailand"},{"name":"Viral Vector Joint Unit and Join Laboratory, Mahidol University, Nakhon Pathom, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wijit","family":"Banlunara","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2120-120X","authenticated-orcid":true,"given":"Aunyaratana","family":"Thontiravong","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"},{"name":"Animal Vector-Borne Disease Research Unit, Veterinary Parasitology Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"},{"name":"Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals (CUEIDAs), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"},{"name":"Center of Excellence of Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10081010"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13230"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2014.973832"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1080\/09712119.2022.2026361"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2021.198374"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018106"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3201\/eid2112.150600"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.101890"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00581"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108636"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-2020-8"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108870"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14402"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.890263"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12859"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12975"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-005-0060-6"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.10.012"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1637\/7732-101006-REGR.1"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1084\/jem.92.5.441"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800068059"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14155"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00978-19"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01257-06"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.02.013"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1668-y"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2022.109500"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14467"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02011-19"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.3390\/v13050921"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00427-18"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.3390\/v14030447"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02331-20"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tbed\/2023\/9239953.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tbed\/2023\/9239953.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tbed\/2023\/9239953.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:23:30Z","timestamp":1701217410000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/tbed\/2023\/9239953\/"}},"subtitle":[],"editor":[{"given":"Horacio","family":"Bach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,11,28]]},"references-count":34,"alternative-id":["9239953","9239953"],"URL":"https:\/\/doi.org\/10.1155\/2023\/9239953","relation":{},"ISSN":["1865-1682","1865-1674"],"issn-type":[{"value":"1865-1682","type":"electronic"},{"value":"1865-1674","type":"print"}],"subject":[],"published":{"date-parts":[[2023,11,28]]}}}