{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T08:29:06Z","timestamp":1767169746535,"version":"build-2238731810"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Planning Project of Baoding City, Hebei Province","award":["1951ZF007"],"award-info":[{"award-number":["1951ZF007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2023,4,14]]},"abstract":"<jats:p>\n                    Objective. This study is to investigate the difference in HIV-1 RNA pol gene expression in AIDS patients before and after antiviral treatment and its effect on the expression level of CD4+\/CD8+ T cells in peripheral blood. Methods. The participants included 200 AIDS patients who had undergone antiviral medication, and the quantity of HIV-1 RNA pol gene was determined using nested polymerase chain reaction (nPCR). The levels of CD3+, CD4+, and CD8+ T lymphocytes in peripheral blood were measured by flow cytometry before and after therapy. The receiver operating characteristics (ROC) curve was used to assess the impact of HIV-1 RNA pol gene expression and the CD4+\/CD8+ ratio on the prognosis of AIDS patients. Results. After three months of therapy, the levels of HIV-1 RNA and viral load in the patients showed a drastic decline, while the levels of CD4+\/CD8+ were markedly elevated (\n                    <jats:inline-formula>\n                      <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                      <\/a:math>\n                    <\/jats:inline-formula>\n                    ). Logistic analysis revealed that patients\u2019 viral loads were positively correlated with HIV-1 RNA and negatively correlated with CD4+\/CD8+ (\n                    <jats:inline-formula>\n                      <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                      <\/c:math>\n                    <\/jats:inline-formula>\n                    ). The alanine aminotransferase (ALT), white blood cell (WBC) count, Serum creatinine (Cr), total cholesterol (TC), triglyceride (TG), and platelet (PLT) levels significantly increased following a 24-month therapy, while no significant changes were observed in the level of aspartate aminotransferase (AST), red blood cell (RBC), and neutrophil (NEU) (%). (\n                    <jats:inline-formula>\n                      <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>P<\/e:mi>\n                        <e:mo>&gt;<\/e:mo>\n                        <e:mn>0.05<\/e:mn>\n                      <\/e:math>\n                    <\/jats:inline-formula>\n                    ). Conclusion. Antiviral drugs significantly inhibit the HIV-1 RNA POL gene expression and viral load in AIDS patients but upregulate the expression level of CD4+\/CD8+ T cells in peripheral blood.\n                  <\/jats:p>","DOI":"10.1155\/2023\/9910542","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T14:20:10Z","timestamp":1681482010000},"page":"1-9","source":"Crossref","is-referenced-by-count":1,"title":["Treatment with Antiviral Drugs Will Significantly Inhibit the HIV-1 RNA POL Gene Expression and Viral Load in AIDS Patients"],"prefix":"10.1155","volume":"2023","author":[{"given":"Penghui","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Baoding People's Hospital, Baoding City, Hebei Province 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Wang","sequence":"additional","affiliation":[{"name":"Baoding People's Hospital, Baoding City, Hebei Province 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaomiao","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Baoding People's Hospital, Baoding City, Hebei Province 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Baoding People's Hospital, Baoding City, Hebei Province 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Baoding People's Hospital, Baoding City, Hebei Province 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3292-0162","authenticated-orcid":true,"given":"Weiguang","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Baoding People's Hospital, Baoding City, Hebei Province 071000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.d01-1826.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1089\/apc.2018.0015"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1080\/17441692.2016.1240822"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S2055-6640(20)30242-9"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3390\/v12040412"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a007161"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191930"},{"key":"8","unstructured":"XingxingT.A Retrospective Study of Traditional Chinese Medicine Intervention on the Adverse Reactions of AIDS HARRT, [Ph.D. thesis]2020Hunan University of Traditional Chinese Medicine"},{"key":"9","unstructured":"ManY.Study on the Effect of Bushen Tuodu and Huayu on Poor Immune Reconstitution after AIDS Antiretroviral Therapy, [Ph.D. thesis]2019Chengdu University of Traditional Chinese Medicine"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.2174\/1570162X17666181205130532"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000001784"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.02002"},{"issue":"3","key":"13","first-page":"779","article-title":"Ocular manifestation and correlation with CD4+ T cell count among adult HIV\/AIDS patients in Bangladesh","volume":"31","author":"S. 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