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BLAST and phylogenetic analyses showed that PRRSV2\/CN\/FJLX06\u20102021 was most closely related to HeN1201 and HuN4 (the parental strain of MLV HuN4\u2010F112) and clustered with Chinese HP\u2010PRRSV strains in PRRSV\u20102 lineage 8.7. Importantly, 29 of the 39 characteristic amino acid mutations in the HuN4\u2010F112 genome were found at the corresponding sites of PRRSV2\/CN\/FJLX06\u20102021. Animal studies showed that piglets infected with PRRSV2\/CN\/FJLX06\u20102021 had a persistent high fever, higher viremia, presence of interstitial pneumonia, and a higher mortality rate (40%) within 2 weeks than those vaccine\u2010inoculated with HuN4\u2010F112. Taken together, these data suggest that PRRSV2\/CN\/FJLX06\u20102021 is an MLV\u2010like strain that has evolved from MLV HuN4\u2010F112 and is highly pathogenic to piglets.<\/jats:p>","DOI":"10.1155\/2024\/1297975","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T12:22:26Z","timestamp":1718972546000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization and Pathogenicity of a Porcine Reproductive and Respiratory Syndrome Virus Strain with Strong Homology to a HP\u2010PRRSV Vaccine Strain in the Field"],"prefix":"10.1155","volume":"2024","author":[{"given":"Chunhua","family":"Wei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Lan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiqin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manlin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4511-4509","authenticated-orcid":false,"given":"Jiankui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,21]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-015-2672-z"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03084-22"},{"key":"e_1_2_9_3_2","first-page":"1","article-title":"Reproductive failure of unknown etiology","volume":"1","author":"Keffaber K. 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