{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:40:15Z","timestamp":1789580415001,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":198,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008233","name":"School of Medicine, Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["JYZZ037"],"award-info":[{"award-number":["JYZZ037"]}],"id":[{"id":"10.13039\/501100008233","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["821RC733"],"award-info":[{"award-number":["821RC733"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Colitis is a refractory intestinal inflammatory disease significantly affecting the quality of a patient\u2019s life and increasing the risk of exacerbation. The primary factors leading to colitis encompass infections, insufficient blood flow, and the buildup of collagen as well as white blood cells. Among various available therapeutics, 5\u2010methoxytryptophan (5\u2010MTP) has emerged as one of the protectants by inhibiting inflammatory damage. Nonetheless, there is no report on the role of 5\u2010MTP in the treatment of colitis. <jats:italic>Materials and Methods<\/jats:italic>. To verify the anti\u2010inflammatory effect of 5\u2010MTP <jats:italic>in vivo<\/jats:italic>, we first constructed mouse model with dextran sulfate sodium\u2010induced colitis. Furthermore, the macrophage infiltration and release of inflammatory factors through western blot (WB) and hematoxylin\u2013eosin staining analyses were examined. Intestinal epithelial cell tight junction damage and apoptosis were investigated by WB analysis, immunofluorescence, and terminal deoxynucleotidyl transferase dUTP nick end labeling staining. Finally, we examined the generation of cellular inflammation and analyzed the influence of 5\u2010MTP on M1 polarization at the cellular level. <jats:italic>Results<\/jats:italic>. This study initially confirmed that 5\u2010MTP possessed an excellent therapeutic effect on colitis. 5\u2010MTP inhibits macrophage infiltration and the generation of inflammatory factors. In addition to its effects on immune cells, 5\u2010MTP significantly inhibits intestinal epithelial cell tight junction damage and apoptosis <jats:italic>in vivo<\/jats:italic>. Moreover, it inhibits inflammation and M1 polarization response <jats:italic>in vitro<\/jats:italic>. <jats:italic>Conclusion<\/jats:italic>. 5\u2010MTP counteracts excessive inflammation, thereby preventing intestinal epithelial tight junction damage. In addition, inhibition of apoptosis suggests that 5\u2010MTP may be a potential therapeutic agent for colitis.<\/jats:p>","DOI":"10.1155\/2024\/1484806","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T10:49:41Z","timestamp":1721213381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["5\u2010Methoxytryptophan Alleviates Dextran Sulfate Sodium\u2010Induced Colitis by Inhibiting the Intestinal Epithelial Damage and Inflammatory Response"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3445-2184","authenticated-orcid":false,"given":"Yanling","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6092-956X","authenticated-orcid":false,"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8026-9557","authenticated-orcid":false,"given":"Qinyuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0258-0460","authenticated-orcid":false,"given":"Zhenhang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4980-0952","authenticated-orcid":false,"given":"Fang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8588-343X","authenticated-orcid":false,"given":"Xiangyan","family":"Ai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7448-218X","authenticated-orcid":false,"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3785-3643","authenticated-orcid":false,"given":"Dongbao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3223-3401","authenticated-orcid":false,"given":"Futao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8891-1401","authenticated-orcid":false,"given":"Peng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,17]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101330"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i29.9872"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-019-2750-y"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/540S97a"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.10.002"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00400"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1517\/14740331003639412"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209919109"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0844fje"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094490"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308559"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-020-00671-w"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152166"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-023-01769-1"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-21-17"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2022-327337"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00059"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.05015-11"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3859"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.06.235"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/152308603770380025"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200207000-00015"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102469"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01081-y"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2024\/1484806","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T10:49:46Z","timestamp":1721213386000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2024\/1484806"}},"subtitle":[],"editor":[{"given":"Qingdong","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1155\/2024\/1484806"],"URL":"https:\/\/doi.org\/10.1155\/2024\/1484806","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1484806"}}