{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:39:53Z","timestamp":1785796793946,"version":"3.56.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T00:00:00Z","timestamp":1719619200000},"content-version":"vor","delay-in-days":180,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Stevia (<jats:italic>Stevia rebaudiana<\/jats:italic>), recognized for its low\u2010calorie, sugar\u2010free attributes, and various health benefits, has potential applications beyond human consumption, particularly in agriculture. This study explored the potential uses of Stevia in both agricultural and healthcare contexts by examining its plant\u2010inhibitory, cytotoxic, and antioxidant effects. The methanolic extract of Stevia leaves was fractionated into hexane, ethyl acetate, chloroform, and water fractions. These fractions were then subjected to the bioassay analyses above and underwent identification of their chemical constituents. The results indicated that the ethyl acetate fraction demonstrated significant inhibitory effects on weed germination and growth of Beggars tick (<jats:italic>Bidens frondosa<\/jats:italic>) (100% inhibition at 1000\u2009ppm of dose). This fraction also exhibited the highest antioxidant activity, total phenolic, and total flavonoid contents (IC<jats:sub>50<\/jats:sub> DPPH\u2009=\u200918.67\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL, 103.50\u2009mg GAE\/g fraction, and 410.16\u2009mg QE\/g fraction, respectively). In contrast, the chloroform fraction showed the highest cytotoxic effect (LC<jats:sub>50<\/jats:sub>\u2009=\u2009700.01\u2009ppm) in the brine shrimp (<jats:italic>Artemia salina<\/jats:italic>) mortality evaluation. Pearson\u2019s correlation analysis revealed a positive correlation among plant inhibitory effects, antioxidant potentials, and phenolic\/flavonoid contents of Stevia. FTIR spectra confirmed the presence of phenols and nonpolar components in the ethyl acetate and chloroform fractions. In addition, GC\u2010MS analysis successfully identified Stevia\u2019s key constituents, including tetracontane, hexadecane, hexadecanoic acid, methyl ester in the ethyl acetate fraction, and spiro [4.5] decan\u20107\u2010one and 6\u2010hydroxy\u20104,4,7a\u2010trimethyl\u20105,6,7,7a\u2010tetrahydrobenzofuran\u20102(4H)\u2010one in the chloroform fraction. This study underscores the potential of <jats:italic>S. rebaudiana<\/jats:italic> as a source of natural antioxidants and herbicides, offering valuable insights into its diverse applications in agriculture.<\/jats:p>","DOI":"10.1155\/2024\/2200993","type":"journal-article","created":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T06:04:02Z","timestamp":1719727442000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Phytotoxic and Cytotoxic Effects, Antioxidant Potentials, and Phytochemical Constituents of <i>Stevia rebaudiana<\/i> Leaves"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0803-0389","authenticated-orcid":false,"given":"Einstivina","family":"Nuryandani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dedy","family":"Kurnianto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmadi","family":"Jasmadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ardiba Rakhmi","family":"Sefrienda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erliana","family":"Novitasari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erni","family":"Apriyati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3431-0504","authenticated-orcid":false,"given":"Yeyen Prestyaning","family":"Wanita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti Dewi","family":"Indrasari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5035-4195","authenticated-orcid":false,"given":"Rofiq","family":"Sunaryanto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donowati","family":"Tjokrokusumo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvi","family":"Yani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indyaswan Tegar","family":"Suryaningtyas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6052-301X","authenticated-orcid":false,"given":"Yusuf","family":"Andriana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,29]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612822666161021142835"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-015-1883-3"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.15228\/anst.2021.v01.i04.p03"},{"key":"e_1_2_9_4_2","first-page":"31","article-title":"Determination of in vitro antimicrobial activity of Stevia rebaudiana (Bertoni) leaf extracts against antibiotic-resistant microorganisms","volume":"23","author":"Afrin S.","year":"2021","journal-title":"Asian Journal of Microbiology, Biotechnology and Environmental Sciences"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2019.108683"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.17179\/excli2021-4211"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3573005"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.07.135"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.09.046"},{"key":"e_1_2_9_10_2","first-page":"163","article-title":"Brine shrimp toxicity study of different Bangladeshi medicinal plants","volume":"4","author":"Rahmatullah M.","year":"2010","journal-title":"Advances in Natural and Appled Sciences"},{"key":"e_1_2_9_11_2","first-page":"593","article-title":"Allelopathic and in vitro anticancer activity of stevia and chia","volume":"26","author":"Dragoeva A.","year":"2017","journal-title":"Chemistry: Bulgarian Journal of Science Education"},{"key":"e_1_2_9_12_2","first-page":"6675","article-title":"Effect of different extracts of callus and plantlets of Stevia rebaudiana (Bertoni) on seed germination of some agricultural crops","volume":"9","author":"Taware A. 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