{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:22:32Z","timestamp":1753888952608,"version":"3.41.2"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":206,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Applied Bionics and Biomechanics"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Moyamoya disease (MMD) leads to nerve injury. Exosomes are touted as bio\u2010shuttles for the delivery of distinct biomolecules inside the cells. Recently, UCH\u2010L1 was shown to play a vital role in nerve injury. However, it is still unknown whether UCH\u2010L1 can improve the nerve injury of MMD. <jats:italic>Materials and Methods<\/jats:italic>. Exosomes were isolated from the serum of patients with MMD and healthy controls. The total RNA was extracted from the exosomes, and the level of GFAP and UCH\u2010L1 between the serum exosomes of the two groups was analyzed by a quantitative reverse transcription\u2010polymerase chain reaction and western blot. Exosome labeling and uptake by SH\u2010SY5Y cells were observed by confocal laser microscopy. Cell counting kit\u20108 assay and flow cytometry were used to determine the viability and apoptosis of SH\u2010SY5Y cells, respectively. <jats:italic>Results<\/jats:italic>. Exosomes were successfully isolated and identified from serum. The expression of GFAP and UCH\u2010L1 was significantly higher in the serum\u2010derived exosomes from MMD patients compared with the healthy controls (<jats:italic>P<\/jats:italic>\u2009 &lt; 0.05). Compared to the blank and control exosome group, serum\u2010derived exosomes from MMD significantly suppress cellular vitality and promote apoptosis of SH\u2010SY5Y cells, while the use of LDN\u201091946, a specific inhibitor of UCH\u2010L1, could reverse the effects induced by serum\u2010derived exosomes from MMD. <jats:italic>Conclusion<\/jats:italic>. UCH\u2010L1 inhibitor could reverse MMD\u2010induced inhibition of SH\u2010SY5Y cell viability and promotion of apoptosis. UCH\u2010L1 may be a therapeutic target for the treatment of nerve damage caused by MMD.<\/jats:p>","DOI":"10.1155\/2024\/2550642","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T11:33:49Z","timestamp":1721907229000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UCH\u2010L1 Inhibitor Alleviates Nerve Damage Caused by Moyamoya 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