{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:46:35Z","timestamp":1740149195725,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T00:00:00Z","timestamp":1706054400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Culture, Research, and Technology, Indonesia","award":["1071.29\/UN10.C10\/TU\/2022","2617\/12.\/UN10.F08\/PN\/2023"],"award-info":[{"award-number":["1071.29\/UN10.C10\/TU\/2022","2617\/12.\/UN10.F08\/PN\/2023"]}]},{"DOI":"10.13039\/501100006374","name":"Universitas Brawijaya","doi-asserted-by":"publisher","award":["1071.29\/UN10.C10\/TU\/2022","2617\/12.\/UN10.F08\/PN\/2023"],"award-info":[{"award-number":["1071.29\/UN10.C10\/TU\/2022","2617\/12.\/UN10.F08\/PN\/2023"]}],"id":[{"id":"10.13039\/501100006374","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Interdisciplinary Perspectives on Infectious Diseases"],"published-print":{"date-parts":[[2024,1,24]]},"abstract":"<jats:p>Introduction. The imbalance of the immune response is an important factor contributing to the incidence of ocular toxoplasmosis (OT). Regulatory T cells (Treg) play a key role in maintaining the balance between Th1 and Th17 immune responses, while interleukin-27 (IL-27) levels are related to the differentiation of Th17\u2009cells. This study analyzes the differences in the number of Treg cells and the level of IL-27 between OT patients and seropositive individuals without ocular lesions and its correlation with retinal lesion size. Methods. This analytic observational study, conducted for 8\u2009months, involved 11 OT patients and 10 seropositive individuals without ocular lesions. All subjects underwent a comprehensive ophthalmological examination. Retinal lesions were documented by fundus photographs and the size was measured using Digimizer 4.2.2.0 software. Isolation of peripheral blood mononuclear cells (PBMC) was performed to measure the number of Treg cells using flow cytometry and interleukin-27 levels were assessed using the Sandwich enzyme-linked immunosorbent assay (ELISA) technique. Data were analyzed with SPSS. Result. The number of Treg cells in the OT group (47.16\u2009\u00b1\u200915.66%) was lower than in the seropositive group without the ocular lesions (62.86\u2009\u00b1\u200917.08%) (<jats:inline-formula><a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><a:mi>p<\/a:mi><a:mo>=<\/a:mo><a:mn>0.029<\/a:mn><\/a:math><\/jats:inline-formula>). The serum IL-27 levels in the OT group were not significantly different from the seropositive group without the ocular lesions (<jats:inline-formula><c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><c:mi>p<\/c:mi><c:mo>=<\/c:mo><c:mn>0.360<\/c:mn><\/c:math><\/jats:inline-formula>). The number of Treg cells was significantly related to retinal lesion size (<jats:inline-formula><e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><e:mi>p<\/e:mi><e:mo>=<\/e:mo><e:mn>0.043<\/e:mn><\/e:math><\/jats:inline-formula>), with a correlation coefficient of \u22120.648, indicating a strong and inverse correlation. There was no significant correlation between serum IL-27 levels and retinal lesion size (<jats:inline-formula><g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><g:mi>p<\/g:mi><g:mo>=<\/g:mo><g:mn>0.556<\/g:mn><\/g:math><\/jats:inline-formula>). Conclusion. Ocular toxoplasmosis patients have a low number of Treg cells that are inversely related to the retinal lesion size. The size of the retinal lesion increases as the number of Treg cells decreases.<\/jats:p>","DOI":"10.1155\/2024\/3495376","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T21:50:08Z","timestamp":1706133008000},"page":"1-7","source":"Crossref","is-referenced-by-count":0,"title":["The Decreased Treg Cells Number Associated with Retinal Lesion Size in Ocular Toxoplasmosis"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8317-7626","authenticated-orcid":true,"given":"Ovi","family":"Sofia","sequence":"first","affiliation":[{"name":"Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia"},{"name":"Department of Ophthalmology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6887-0181","authenticated-orcid":true,"given":"Muna","family":"Amalia","sequence":"additional","affiliation":[{"name":"Residency Training Program, Department of Ophthalmology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6277-9818","authenticated-orcid":true,"given":"Herryanto","family":"Thomassawa","sequence":"additional","affiliation":[{"name":"Residency Training Program, Department of Ophthalmology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4880-1048","authenticated-orcid":true,"given":"Loeki Enggar","family":"Fitri","sequence":"additional","affiliation":[{"name":"Department of Clinical Parasitology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7864-3001","authenticated-orcid":true,"given":"Seskoati","family":"Prayitnaningsih","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4019-9109","authenticated-orcid":true,"given":"Hani","family":"Susianti","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"volume-title":"Advances in Ocular Toxoplasmosis. Uveitis: An Update","year":"2016","author":"A. Braakenburg","key":"1"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00636-19"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2013.12.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1055\/s-0041-111821"},{"issue":"5","key":"5","first-page":"67","article-title":"Determinan lingkungan dan perilaku berhubungan dengan terjadinya penyakit infeksi toxoplasmosis di wilayah kota Semarang","volume":"4","author":"N. Aditama","year":"2016","journal-title":"Jurnal Kesehatan Masyarakat (Undip)"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.35479\/ijretina.2019.vol002.iss002.96"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2016.06.053"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1080\/09273948.2021.1922706"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100882"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1086\/597478"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018139"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.09.007"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/s1074-7613(03)00300-5"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.10.001"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.04.004"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw041"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2022.02.028"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2014-305843"},{"issue":"2","key":"19","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1017\/S0950268899002800","article-title":"Incidence of symptomatic toxoplasma eye disease: aetiology and public health implications","volume":"123","author":"R. 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Dupouy-Camet","year":"2012","journal-title":"OD Toxoplasmosis-Recent Advances Publisher: InTech Risk Factors Pathogenesis and Diagnosis of Ocular Toxoplasmosis"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.03.005"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1590\/s0074-02762009000200014"}],"container-title":["Interdisciplinary Perspectives on Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ipid\/2024\/3495376.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ipid\/2024\/3495376.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ipid\/2024\/3495376.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T21:50:16Z","timestamp":1706133016000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ipid\/2024\/3495376\/"}},"subtitle":[],"editor":[{"given":"Payam","family":"Behzadi","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,1,24]]},"references-count":33,"alternative-id":["3495376","3495376"],"URL":"https:\/\/doi.org\/10.1155\/2024\/3495376","relation":{},"ISSN":["1687-7098","1687-708X"],"issn-type":[{"type":"electronic","value":"1687-7098"},{"type":"print","value":"1687-708X"}],"subject":[],"published":{"date-parts":[[2024,1,24]]}}}