{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T22:52:57Z","timestamp":1776466377937,"version":"3.51.2"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":172,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015758","name":"Adama Science and Technology University","doi-asserted-by":"publisher","award":["ASTU\/AS-R\/003\/2020"],"award-info":[{"award-number":["ASTU\/AS-R\/003\/2020"]}],"id":[{"id":"10.13039\/501100015758","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry Research International"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>The plant <jats:italic>Hydnora johannis<\/jats:italic> has been utilized in folk medicine. Analyzing phytochemical composition of dichloromethane\/methanol (1\u2009:\u20091) root part of <jats:italic>Hydnora johannis<\/jats:italic> gave oleic acid (<jats:bold>1<\/jats:bold>), caffeic acid\u20102\u2010hydroxynonylester (<jats:bold>2<\/jats:bold>), catechin (<jats:bold>3<\/jats:bold>), and a pregnane derivative (<jats:bold>4<\/jats:bold>). NMR spectroscopy was used to characterize compounds <jats:bold>1\u20133<\/jats:bold>, while compound <jats:bold>4<\/jats:bold> was identified through GC\u2010MS analysis and literature comparison. The cytotoxicity of extracts from roots of <jats:italic>H. johannis<\/jats:italic> was conducted against MCF\u20107\u2009cell lines (human breast cancer) by MTT assay. According to the cytotoxicity study, <jats:italic>n<\/jats:italic>\u2010hexane extract exhibited a high level of toxicity with 28.9\u2009\u00b1\u20095.6%\u2009cell viability. Antibacterial activity was tested against <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, <jats:italic>Staphylococcus aureus<\/jats:italic>, and <jats:italic>Streptococcus pyogen.<\/jats:italic> The highest bacterial growth mean inhibition zone was measured for catechin (3) (13.72\u2009\u00b1\u20090.05\u2009mm)) against <jats:italic>P. aeruginosa<\/jats:italic> at 0.25\u2009mg\/mL and acceptable related to standard. Antioxidant activity was studied by the DPPH assay. Based on the data from the antioxidant study, DCM\/MeOH extract (70.32%) and catechin (<jats:bold>3<\/jats:bold>) showed good antioxidant activity (65.61%) (IC<jats:sub>50<\/jats:sub> 0.25\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) relative to that of the positive control (78.21%, IC<jats:sub>50<\/jats:sub> 0.014\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) at 12.5\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. In each docking pose, catechin (<jats:bold>3<\/jats:bold>) scored higher binding affinity of \u22127.9, \u22127.2, and \u22126.4\u2009kcal\/mol towards PqsA, DNA gyraseB, and <jats:italic>S. aureus<\/jats:italic> PK, respectively, compared to amoxicillin (\u22128.1, \u22126.1, and \u22126.4\u2009kcal\/mol). All five Lipinski rules were obeyed by compounds <jats:bold>1\u20133<\/jats:bold>, which showed an acceptable drug resemblance. The lipophilicity was computed as less than five (1.47\u20134.01) indicating a lipophilic property. Catechin (<jats:bold>3<\/jats:bold>) obeys Veber\u2019s rule implying its good oral bioavailability. Binding affinity scores of catechin (<jats:bold>3<\/jats:bold>)\u2010protein interactions are in line with those from <jats:italic>in vitro<\/jats:italic> tests, indicating its potential antibacterial effect. The obtained cytotoxicity and antibacterial activity results support the utilization of <jats:italic>H. johannis<\/jats:italic> in folk medicine.<\/jats:p>","DOI":"10.1155\/2024\/3713620","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T12:31:57Z","timestamp":1718973117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>In Vitro<\/i> Antibacterial, Antioxidant, Cytotoxicity Activity, and <i>In Silico<\/i> Molecular Modelling of Compounds Isolated from Roots of <i>Hydnora johannis<\/i>"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0369-2474","authenticated-orcid":false,"given":"Teshome","family":"Degfie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5301-9923","authenticated-orcid":false,"given":"Milkyas","family":"Endale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5822-079X","authenticated-orcid":false,"given":"Muhdin","family":"Aliye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8331-2100","authenticated-orcid":false,"given":"Rajalakshmanan","family":"Eswaramoorthy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9256-1302","authenticated-orcid":false,"given":"Tariku Nefo","family":"Duke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4767-606X","authenticated-orcid":false,"given":"Aman","family":"Dekebo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,21]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/ppp3.9"},{"key":"e_1_2_9_2_2","first-page":"222","article-title":"Ethnobotany, chemical constituents and biological activities of the flowers of Hydnora abyssinica A. 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